Arabidopsis FOX lines (rice cDNA)

Resource number: pss11001-pss11004
Developer:  Dr. Minami Matsui (RIKEN PSC)
Total number of line: approx. 20,000 (expected)
Style of distribution: Provided as a seed pool set (seed pool contains approx. 400 seeds from 50 lines = 8 seeds per line; one seed pool set contains 20 seed pools = equivalent with 1,000 lines). Seeds for individual lines (psx) that constitute specific seed pool (psp) are available for the users who obtained desired mutant in the seed pool.
Number of pool sets available: 4 seed pool set (equivalent with 3,943 lines*)
(*The pss11004 contains 19 seed pools that are equivalent with 943 lines.)
Background ecotype: Columbia
Vector for transformation with Agrobacterium: pBIG2113SF(pBI121 derivative)
Selection marker: hygromycin
Specific feature: Provided as seed pools for screening. Rice full-length cDNA was introduced with 35S promoter to produce dominant phenotype.
Specific conditions for distribution:
  1. The RECIPIENT agrees to expressly describe that “the BIOLOGICAL RESOURCE (the resource name) was developed by the RIKEN PSC, NIAS and RIBS through The Special Coordination Fund for Promoting Science and Technology, and provided by the RIKEN BRC through the National BioResource Project of the MEXT/AMED, Japan” in Materials and Methods or in the Acknowledgement of any publication reporting the use thereof. The RECIPIENT also agrees to send a copy of such publication to the RIKEN BRC. The RIKEN BRC, RIKEN PSC, NIAS and RIBS may disclose publicly, copy or otherwise use such publication to demonstrate the contribution by the RIKEN BRC.
Kondou Y, Higuchi M, Takahashi S, et al. Systematic approaches to using the FOX hunting system to identify useful rice genes. Plant J. 2009;57(5):883-894. doi:10.1111/j.1365-313X.2008.03733.x PMID: 18980645
Requirements for the safe handling, storage, transport and use:
  1. This BIOLOGICAL MATERIAL is the seed of Arabidopsis thaliana.
  2. This BIOLOGICAL MATERIAL harbor HPT genes (from E. coli), promoter of CaMV 35S RNA genes (from cauliflower mosaic virus), NOS-terminators and T-DNA border sequences (from Agrobacterium tumefaciens), β-lactamase (AmpR), ColE1, Lac promoter (from E.coli), T3 promoter (Enterobacteria phage T3), T7 promoter (Enterobacteria phage T7), F1 ori (Inoviridae filamentous bacteriophage F1), full-length cDNA (from Oryza sativa).
  3. This BIOLOGICAL MATERIAL is for laboratory use (contained use) only. Do not use it for “Intentional introduction into the environment”. This BIOLOGICAL MATERIAL and its progeny should be inactivated by autoclave or equivalent procedure before disposed as a waste.

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