| Resource number: |
pss10001-10021 |
| Developer: |
Dr. Minami Matsui and Dr. Takanari Ichikawa (RIKEN PSC) |
| Total number of line: |
20,494 |
| Style of distribution: |
Provided as a seed pool set (seed pool contains approx. 400 seeds from 50 lines = 8 seeds per line; one seed pool set contains 20 seed pools = equivalent with 1,000 lines). Seeds for individual lines (psx) that constitute specific seed pool (psp) are available for the users who obtained desired mutant in the seed pool. We also distribute seeds of individual lines that have insert cDNA information |
| Number of lines available: |
21 seed pool sets (equivalent with 20,494 lines. The pss10010 contains 16 seed pools and pss10021, 14 seed pools. Others contain 20 seed pools.) |
| Background ecotype: |
Columbia |
| Vector for transformation with Agrobacterium: |
pBIG2113SF(pBI121 derivative) |
| Selection marker: |
hygromycin |
| Specific feature: |
Provided as seed pools for screening. Arabidopsis full-length cDNA (RAFL) was introduced with 35S promoter to produce dominant phenotype. |
| Specific conditions for distribution: |
- When the RECIPIENT has filed a patent application on the invention conceived or made during the course of research studies directly using the BIOLOGICAL RESOURCE, the RECIPIENT agrees to promptly notify RIKEN Plant Science Center upon filing the application with specifications.
- When the RECIPIENT publishes his/her research, acknowledge RIKEN PSC and refer designated manuscripts shown below.
(For the detailed information, please email to plant.brc@riken.jp )
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| Reference: |
| Ichikawa T, Nakazawa M, Kawashima M, Iizumi H, Kuroda H, Kondou Y, Tuhara Y, Suzuki K, Ishikawa A, Seki M, Fujita M, Motohashi R, Nagata N, Takagi T, Shinozaki K and Matsui M (2006) Plant J. 48:974-985 PMID: 17227551 |
| Requirements for the safe handling, storage, transport and use: |
- This BIOLOGICAL MATERIAL is the seed of Arabidopsis thaliana.
- This BIOLOGICAL MATERIAL harbor T-DNA region {HPT gene (resistant to Hygromycin)(from Escherichia coli), CAMV 35S promoter (from cauliflower mosaic virus), NOS promoter, NOS terminator, gene7 promoter, T-DNA border sequences (from Agrobacterium tumefaciens), full-length cDNA (from Arabidopsis thaliana), modified pBluescript < Bla(resistant to Ampicillin)(β-lactamase gene), ColE1 ori, Lac promoter (from Escherichia coli), f1 ori (from Enterobacteriaphage F1), T7 promoter (from Enterobacteriaphage T7), and T3 promoter (from Enterobacteriaphage T3) >} and Vector backbone region {TetR (resistant to Tetracycline), NPTIII(resistant to Kanamycin), oriT, traJ, oriV, IS1, trfA (from Escherichia coli)}. These nucleotides are located on the vector pBIG2113SF derivative.
- This BIOLOGICAL MATERIAL is for laboratory use (contained use) only. Do not use it for “Intentional introduction into the environment”.
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| This BIOLOGICAL MATERIAL and its progeny should be inactivated by autoclave or equivalent procedure before disposed as a waste. |
