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Arabidopsis transposon-tagged lines

Resource number:  pst00001-pst22132 (individual line), pss20001 (seed pool set of homozygous lines for screening), 20002
Developer:  Dr Kazuo Shinozaki, Plant Functional Genomics Research Group, RIKEN GSC.
Total number of line:  17,457 ungenotyped lines plus 3,119 confirmed homozygous lines
Style of distribution:  Individual line (approx. 50 seeds) / Seed pool set of homozygous lines (equivalent with 1,000 lines, a list for 20 lines mixed in each pool (psp20001-20100) is here)
Number of lines available:  17,457 ungenotyped lines plus 3,119 confirmed homozygous lines
Background ecotype:  No-0 (Nossen)* *Please read IMPORTANT NOTE 4.
Vector for transformation with Agrobacterium:  pCGN derivatives
Selection marker:  hygromycin
Specific feature:  Flanking sequences of transposon have been characterized. Sequences for Ds specific primers are here.
Specific conditions for distribution:
(a)The RECIPIENT agrees to expressly describe that “the Arabidopsis transposon-tagged line was developed by the plant genome project of RIKEN Genomic Sciences Center” in any publication reporting the use thereof.
(b)The manuscripts listed below should be referred in the publication on scientific journal.(1)Ito T, Motohashi R, Kuromori T, Mizukado S, Sakurai T, Kanahara H, Seki M and Shinozaki K (2002) A new resource of locally transposed Dissociation elements for screening gene-knockout lines in silico on the Arabidopsis genome. Plant Physiol. 129: 1695-1699.(2)Kuromori T, Hirayama T, Kiyosue Y, Takabe H, Mizukado S, Sakurai T, Akiyama K, Kamiya A, Ito T and Shinozaki K (2004) A collection of 11800 single-copy Ds transposon insertion lines in Arabidopsis. Plant J. 37, 897-905.
  1. Ito T, Motohashi R, Kuromori T, Mizukado S, Sakurai T, Kanahara H, Seki M and Shinozaki K (2002) A new resource of locally transposed Dissociation elements for screening gene-knockout lines in silico on the Arabidopsis genome. Plant Physiol. 129: 1695-1699.(2)Kuromori T, Hirayama T, Kiyosue Y, Takabe H, Mizukado S, Sakurai T, Akiyama K, Kamiya A, Ito T and Shinozaki K (2004) A collection of 11800 single-copy Ds transposon insertion lines in Arabidopsis. Plant J. 37, 897-905.
  2. Kuromori T, Hirayama T, Kiyosue Y, Takabe H, Mizukado S, Sakurai T, Akiyama K, Kamiya A, Ito T and Shinozaki K (2004) A collection of 11800 single-copy Ds transposon insertion lines in Arabidopsis. Plant J. 37, 897-905.
References:
  1. Fedoroff NV. and Smith D. (1993) A versatile system for detecting transposition in Arabidopsis. Plant J. 3: 273-289.
  2. Smith D., Yanai Y., Liu, Y-G., Ishiguro S., Okada K., Shibata D., Whittier RF. and Fedoroff NV. (1996) Characterization and mapping of Ds-GUS-T-DNA lines for targeted insertional mutagenesis. Plant J. 10: 721-732.
  3. Ito T., Motohashi R., Kuromori T., Mizukado S., Sakurai T., Kanahara H., Seki M. and Shinozaki K. (2002) Plant Physiol. 129: 1695-1699.
  4. Kuromori T., Hirayama T., Kiyosue Y., Takabe H., Mizukado S., Sakurai T., Akiyama K., Kamiya A., Ito T. and Shinozaki K. (2004) Plant J. 37, 897-905.
  5. Ito T., Motohashi R., Kuromori T., Noutoshi Y., Seki M., Kamiya A., Mizukado S., Sakurai T. and Shinozaki K. (2005) Plant Cell Physiol. 46: 1149-1153.
IMPORTANT NOTE:
  1. AGI codes are frequently changed. Before you make your order, we recommend you to obtain detail information including flanking sequence of the line from RARGE (Riken Arabidopsis Genome Encyclopedia) or T-DNA express.
  2. The lines marked ‘In preparation’ are currently unavailable. Do not include those lines in your order. We will make an announcement at the time they are ready for distribution.
  3. The transposon-tagged lines are categorized to genetically modified organisms. For ordering such materials, the applicants must obtain all of the permission required by any kind of regulation or law before ordering. Also, it is recipient’s responsibility to perform customs procedure for importation of biological materials. Please inform us when you need quarantine certification to import Arabidopsis seeds. Without providing such information, RIKEN BRC cannot guarantee any loss or damage of the materials that is caused during customs procedure.
  4. Genotypes of two Ds donor lines, namely Ds2-389-2 and Ds6-393-19, were inconsistent with that of Nossen. None of the natural accessions preserved in RIKEN BRC has same genotype with these lines. (These lines were crossed with Ac donor line to produce Ds12- and Ds16- lines.) We recommend users to use the parental lines available from ABRC as control plants in your experiments. List of parental line is here. RIKEN BRC also provides the lines upon your request.
NOTE: Only those lines listed on our catalogue are available. Other lines will be open for distribution after multiplication in RIKEN BRC. For detail, please send your question to: plant.brc@riken.jp


 

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