Arabidopsis Transcription Factor-Glucocorticoid Receptor (TF-GR) lines

Resource number: psg00001-psg05563
Developer: Dr Minami Matsui, Center for Sustainable Resource Science (CSRS) RIKEN 
Total number of line: 5,648 
Style of distribution: Individual line (approx. 50 or 400 seeds)
Number of lines available: 2,439
Background ecotype: Col-0
Vector for transformation with Agrobacterium: pGWTF-GR vector
Selection marker: Kanamycin
Specific conditions for distribution:
1. Citation of designated manuscript in user’s publications required. A collection of inducible transcription factor-glucocorticoid receptor fusion lines for functional analyses in Arabidopsis thaliana. Setsuko Shimada, Yuki Yanagawa, Takachika Munesada, Yoko Horii, Tomoko Kuriyama, Mika Kawashima, Youichi Kondou, Takeshi Yoshizumi, Nobutaka Mitsuda, Masaru Ohme-Takagi, Yuko Makita, Minami Matsui. Plant J. 2022 May 5. doi: 10.1111/tpj.15796 PMID: 35510416 DOI: 10.1111/tpj.15796
2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
3. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
References:
  1. Shimada S, Yanagawa Y, Munesada T, Horii Y, Kuriyama T, Kawashima M, Kondou Y, Yoshizumi T, Mitsuda N, Ohme-Takagi M, Makita Y, Matsui M. A collection of inducible transcription factor-glucocorticoid receptor fusion lines for functional analyses in Arabidopsis thaliana. Plant J. 2022 Jul;111(2):595-607. doi: 10.1111/tpj.15796. Epub 2022 May 21. PMID: 35510416.
Requirements for the safe handling, storage, transport and use:
  1. This BIOLOGICAL MATERIAL is the seed of Arabidopsis thaliana.
  2. This BIOLOGICAL MATERIAL harbor NPTII gene (from E.coli), att sequence (from Enterobacteria phage lambda), promoter of CaMV 35S RNA gene (from cauliflower mosaic virus), Glucocorticoid receptor (from Rat), LB, RB, NOS-promoter and NOS-terminator sequences of T-DNA (Agrobacterium tumefaciens).
  3. This BIOLOGICAL MATERIAL is for laboratory use (contained use) only. Do not use it for “Intentional introduction into the environment”.
IMPORTANT NOTE:
  1. AGI codes are frequently changed. Before you make your order, we recommend you to obtain detail information including inserted sequence of the line from Exp-Plant Catalog.
  2. The lines marked ‘In preparation’ are currently unavailable. Do not include those lines in your order.
  3. The TF-GR lines are categorized to genetically modified organisms. For ordering such materials, the applicants must obtain all of the permission required by any kind of regulation or law before ordering. Also, it is recipient’s responsibility to perform customs procedure for importation of biological materials. Please inform us when you need quarantine certification to import Arabidopsis seeds. Without providing such information, RIKEN BRC cannot guarantee any loss or damage of the materials that is caused during customs procedure.
NOTE: Only those lines listed on our catalogue are available. Other lines will be open for distribution after multiplication in RIKEN BRC. For detail, please send your question to: plant.brc@riken.jp




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