Arabidopsis activation (T-DNA)-tagged lines

Resource number: pss00001-pss00009, developed by RIKEN BRC (The pss00009 contains 17 seed pools. Others contain 20 seed pools.); pss00101-00128, developed by RIKEN GSC (The pss00103 contains 16 seed pools. Others contain 20 seed pools.).
Developer: Dr. Kazuo Shinozaki (Plant Molecular Biology Laboratory, RIKEN Tsukuba Institute), Dr Masatomo Kobayashi (RIKEN BRC), Dr. Minami Matsui (RIKEN GSC)
Total number of line: approx. 70,000
Style of distribution: Provided as a seed pool set (seed pool contains approx. 400 seeds from 50 lines = 8 seeds per line; one seed pool set contains 20 seed pools = equivalent with 1,000 lines). Seeds for individual lines (psa and psm) that constitute specific seed pool (psp) are available for the users who obtained desired mutant in the seed pool. We also distribute seeds for individual mutant lines (psm) that showed interesting phenotypes.
Number of pool sets available: 37 seed pool sets (equivalent with 36,650 lines)
Background ecotype: Columbia
Vector for transformation with Agrobacterium: pPCVICEn4HPT
Selection marker: hygromycin
Specific feature: Provided as seed pools for screening. The 4x35S enhancer in T-DNA promotes gene expression near T-DNA insertion site to produce dominant phenotype.
Specific conditions for distribution:
(a)The RECIPIENT agrees to expressly describe that “the Arabidopsis activation-tagged line was developed by the plant genome project of RIKEN Genomic Sciences Center” in any publication reporting the use thereof.
(b)The manuscript listed below should be referred in the publication on scientific journal.
  1. Nakazawa M, Ichikawa T, Ishikawa A, Kobayashi H, Tsuhara Y, Kawashima M, Suzuki K, Muto S and Matsui M (2003) Activation tagging, a novel tool to dissect the functions of a gene family. Plant Journal 34: 741-750.
Nakazawa M., Ichikawa T., Ishikawa A., Kobayashi H., Tsuhara Y., Kawashima M., Suzuki K., Muto S., Matsui M. (2003) Activation tagging, a novel tool to dissect the functions of a gene family. Plant Journal 34(5): 741-750.

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