Arabidopsis TAC clones

This resource has been developed and deposited from Dr. Daisuke Shibata (Kazusa DNA Research Institute).

Resource number: pdg00001-pdg10752
Developer: Dr. Daisuke Shibata (Kazusa DNA Research Institute)
Total number of Clones: 10,752
Style of distribution: Approx. 100 ng of lyophilized plasmid DNA is provided.
Number of clones available: 10,752
Organism: Arabidopsis thaliana accession Columbia
Vector: pYLTAC7
Selection marker: kanamycin (for culturing E. coli and Agrobacterium) and hygromycin (for selecting transformed plants)
Map and restriction site: HindIII, map and sequence for cloning site are available from Developer’s site
Specific feature: End-sequences of both NotI and SfiI sites have been characterized.
Specific conditions for distribution: Following manuscript should be referred in the publication on a scientific journal.1. Liu YG, Shirano Y, Fukaki H, Yanai Y, Tasaka M, Tabata S, Shibata D. Complementation of plant mutants with large genomic DNA fragments by atransformation-competent artificial chromosome vector accelerates positionalcloning. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6535-40. PubMed PMID:10339623
Reference: 1. Shibata D, Liu YG. Agrobacterium-mediated plant transformation with large DNA fragments. Trends Plant Sci. 2000 Aug;5(8):354-7. PubMed PMID:10908881

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