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Arabidopsis thaliana Arabidopsis thaliana
Brachypodium Brachypodium distachyon
populus Populus nigra
oryza Oryza sativa
lotus Lotus japonicus
lycopersicom Lycopersicon esculentum Mill
striga Striga hermonthica
nicotiana Nicotiana tabacum
(BY-2 cell)
brassica Brassica rapa
thellungiella Thellungiella halophila
physcomitrella Physcomitrella patens
manihot Manihot esculenta Crantz

Arabidopsis TAC clones

This resource has been developed and deposited from Dr. Daisuke Shibata (Kazusa DNA Research Institute).

Resource number: pdg00001-pdg10752
Developer: Dr. Daisuke Shibata (Kazusa DNA Research Institute)
Total number of Clones: 10,752
Style of distribution: Approx. 100 ng of lyophilized plasmid DNA is provided.
Number of clones available: 10,752
Organism: Arabidopsis thaliana accession Columbia
Vector: pYLTAC7
Selection marker: kanamycin (for culturing E. coli and Agrobacterium) and hygromycin (for selecting transformed plants)
Map and restriction site: HindIII, map and sequence for cloning site are available from Developer’s site
Specific feature: End-sequences of both NotI and SfiI sites have been characterized.
Specific conditions for distribution: Following manuscript should be referred in the publication on a scientific journal.1. Liu YG, Shirano Y, Fukaki H, Yanai Y, Tasaka M, Tabata S, Shibata D. Complementation of plant mutants with large genomic DNA fragments by atransformation-competent artificial chromosome vector accelerates positionalcloning. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6535-40. PubMed PMID:10339623
Reference: 1. Shibata D, Liu YG. Agrobacterium-mediated plant transformation with large DNA fragments. Trends Plant Sci. 2000 Aug;5(8):354-7. PubMed PMID:10908881