This resource has been developed and deposited from Dr. Daisuke Shibata (Kazusa DNA Research Institute).
| Resource number: | pdg00001-pdg10752 |
| Developer: | Dr. Daisuke Shibata (Kazusa DNA Research Institute) |
| Total number of Clones: | 10,752 |
| Style of distribution: | Approx. 100 ng of lyophilized plasmid DNA is provided. |
| Number of clones available: | 10,752 |
| Organism: | Arabidopsis thaliana accession Columbia |
| Vector: | pYLTAC7 |
| Selection marker: | kanamycin (for culturing E. coli and Agrobacterium) and hygromycin (for selecting transformed plants) |
| Map and restriction site: | HindIII, map and sequence for cloning site are available from Developer’s site |
| Specific feature: | End-sequences of both NotI and SfiI sites have been characterized. |
| Specific conditions for distribution: | Following manuscript should be referred in the publication on a scientific journal.1. Liu YG, Shirano Y, Fukaki H, Yanai Y, Tasaka M, Tabata S, Shibata D. Complementation of plant mutants with large genomic DNA fragments by atransformation-competent artificial chromosome vector accelerates positionalcloning. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6535-40. PubMed PMID:10339623 |
| Reference: | 1. Shibata D, Liu YG. Agrobacterium-mediated plant transformation with large DNA fragments. Trends Plant Sci. 2000 Aug;5(8):354-7. PubMed PMID:10908881 |
