Experimental Plant Division

This resource is called as “Arabidopsis Transcription Factor clone” (TF clone) that has been developed by National Institute of Advanced Industrial Science and Technology (AIST), RIKEN Center for Sustainable Resource Science (CSRS) and RIKEN Plant Science Center (PSC).

Resource number:	pda90001- (AIST clone) pda91001- (CSRS clone) pda99001- (PSC clone, formerly called as RARTF clone)
Developer:	Drs. Nobutaka Mitsuda and Masaru Takagi (AIST), and Drs. Minami Matsui, Miki Fujita and Kazuo Shinozaki (RIKEN CSRS).
Total number of clones:	2,396
Style of distribution:	Approx. 100 ng of dried plasmid DNA is provided.
Number of available clones:	(AIST clone) 717 (CSRS clone) 1,279 (PSC clone) 400
Background ecotype:	Columbia
Vector:	(AIST/CSRS clone) pENTR207 (PSC clone) pENTR/D/TOPO
Selection marker:	(AIST/CSRS clone) Gentamycin (PSC clone) Kanamycin
Map and restriction site:	Please see the figure in supplier’s catalogue.
Specific feature:	(AIST/CSRS clone) ORF clones without stop codon. (PSC clone) Fully sequenced ORF clones without stop codon.
Specific conditions for distribution:
(CSRS clone) 1. The BIOLOGICAL RESOURCE can be used only for academic research. 2. The number of the BIOLOGICAL RESOURCE distributed to the RECIPIENT is less than 100 in each fiscal year. When the RECIPIENT needs more than 100 clones, the RECIPIENT is required to discuss with the RIKEN BRC and the DEPOSITOR. 3. The RECIPIENT needs to maintain the BIOLOGICAL RESOURCE only in their laboratory and cannot re-distribute to the third party.   The manuscripts listed below should be referred in the publication on scientific journal: (AIST clone) 1. Mitsuda N, Ikeda M, Takada S, Takiguchi Y, Kondou Y, Yoshizumi T, Fujita M, Shinozaki K, Matsui M, Ohme-Takagi M. Efficient yeast one-/two-hybrid screening using a library composed only of transcription factors in Arabidopsis thaliana. Plant Cell Physiol. 2010; 51(12):2145-51. (PSC clone) 1. Iida K, Seki M, Sakurai T, Satou M, Akiyama K, Toyoda T, Konagaya A, Shinozaki K. RARTF: database and tools for complete sets of Arabidopsis transcription factors. DNA Res. 2006; 12:247-56.
Reference:
(AIST/CSRS clone) 1. Mitsuda N, Ikeda M, Takada S, Takiguchi Y, Kondou Y, Yoshizumi T, Fujita M, Shinozaki K, Matsui M, Ohme-Takagi M. Efficient yeast one-/two-hybrid screening using a library composed only of transcription factors in Arabidopsis thaliana. Plant Cell Physiol. 2010; 51(12):2145-51. (PSC clone) 1. Iida K, Seki M, Sakurai T, Satou M, Akiyama K, Toyoda T, Konagaya A, Shinozaki K. RARTF: database and tools for complete sets of Arabidopsis transcription factors. DNA Res. 2006; 12:247-56.
NOTE:
1. The AGI code and gene model applied to the catalogue depend on the Araport 11. 2. The open reading frame is in frame with attL1 sequence and does not contain stop codon unless notified in the Comments. 3. Please examine the sequence of clones on the catalogue prior to make your order. 4. Gateway® is a registered trademark of Invitrogen.