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Brachypodium embryogenic callus (domestic only)

Resource number: rpc10001

Species name: Brachypodium distachyon accession Bd21

Starting material: Immature embryos

Characteristic feature: Embryogenic callus suitable for the regeneration of transgenic plants by using the Agrobacterium-mediated method or the particle bombardment technique

Culture conditions: Callus induction medium 2, 27℃ (lower limit 25℃), dark

Method for maintenance: Transfer a portion (approx. 2 mm in diameter) of growing callus to a fresh medium every 10 days. The growth of callus may be much slower under lower temperature such as 25℃. If it is the case, you would better wait for 14 days untill next subculture.

Protocols (in Japanese): Handling techniques – Brachpodium distachyon

  • Transformation protocol, including culture media preparation
  • Handling of cultured cells after arrival

    • Reference: Alves S, Worland B, Thole V, Snape JW, Bevan MW, Vain P (2009) A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21. Nature Protocols 4: 638–649. DOI: 10.1038/nprot.2009.30

    Specific condition in MTA: None

    Note: We recommend recipients to use the callus for transformation within one month after its arrival.



     

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