Species name: Embryogenic callus of Brachypodium distachyon
Resource Number: rpc10001
Species: Brachypodium distachyon
Line name: Bd21
Characteristic feature: Able to use for transformation using Agrobacterium / particle bombardment
Medium: Callus induction medium 2
Method for maintenance: Transfer a portion (approx. 2mm in diameter) of growing callus to a fresh medium every 10 days. The growth of callus may be much slower under lower temperature such as 25℃. If it is the case, you would better wait for 14 days untill next subculture.
Temperature condition: 27℃ (Lower limit: 25℃)
Light condition: Dark
Reference: Nat. Protoc. 4, 638-649 (2009)
Specific condition in MTA: none
Note: We recommend recipients to use the callus for transformation within one month after its arrival.