Database: Exp-Plant Catalog – Plant Cell Lines
On-line manual: RIKEN BRC plant cell line documentation
Plant cell line collection
Experimental Plant Division provides researchers with plant cell cultures that are useful for a wide range of plant research.
- Cultured cells of model plants
- Cell lines derived from secondary metabolite-producing plants
- Transgenic cell lines expressing fluorescent proteins
Most of the cell lines are undifferentiated cell cultures that have lost capacity for differentiating into whole plants, except for carrot kurodagosun (rpc00002).
Culture methods
The plant cell cultures have been maintained on semi-solid or liquid media by repeated subculturing at definite time intervals.
Detailed culture methods are found in an on-line manual RIKEN BRC plant cell line documentation.
Ordering
Tobacco BY-2 and Arabidopsis T87
Please send MTA and order form to customer support plant.brc@riken.jp.
Documents required for distribution <Tobacco BY-2 / Arabidopsis T87 cells>
Other cell lines
Please contact customer support plant.brc@riken.jp.
Shipping
Setting up schedule to ship orders is affected by the subculture cycle of plant cells or certain terms and conditions when they are shipped.
- Recipients are solely responsible for the customs clearance in accordance with laws of own country.
Asking your local courier for advice would be recommended. - Plant cells are shipped by means of consignee payment.
FedEx is exclusively selected for the recipients in the States, but another choice is available in other areas. - There are several transgenic cell lines in the stock, which cannot regenerate into whole plants under natural conditions.
If any document relating to GMOs is required in the country’s law and/or the institute, please give the customer support a prior notice.
Delivering
Carefully handle the plant cells after they arrive.
- Be prepared with growth chambers, cell culture instruments and culture media.
- Transfer the cultured cells to fresh medium immediately.
- Storing the cultured cells in a refrigerator or a freezer must be avoided.
Quality control
Experimental Plant Division has checked plant cell lines by PCR-based genotyping in order to authenticate their identity.
- Identification of the original plant species (once a year)
- Detection of inserted DNA (if any, once a year)
Citation of cell lines
When results obtained by using the plant cell lines are published in a scientific journal, it should be cited in the following manner:
Nicotiana tabacum BY-2 cell line (rpc00001) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan.
