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Resource List

Arabidopsis thaliana Arabidopsis thaliana
Brachypodium Brachypodium distachyon
populus Populus nigra
oryza Oryza sativa
lotus Lotus japonicus
lycopersicom Lycopersicon esculentum Mill
striga Striga hermonthica
nicotiana Nicotiana tabacum
(BY-2 cell)
brassica Brassica rapa
thellungiella Thellungiella halophila
physcomitrella Physcomitrella patens
manihot Manihot esculenta Crantz

Arabidopsis FOX lines (Arabidopsis cDNA)

Resource number: pss10001-10021
Developer:  Dr. Minami Matsui and Dr. Takanari Ichikawa (RIKEN PSC)
Total number of line: 20,496
Style of distribution: Provided as a seed pool set (seed pool contains approx. 400 seeds from 50 lines = 8 seeds per line; one seed pool set contains 20 seed pools = equivalent with 1,000 lines). Seeds for individual lines (psx) that constitute specific seed pool (psp) are available for the users who obtained desired mutant in the seed pool. We also distribute seeds of individual lines that have insert cDNA information
Number of lines available: 21 seed pool sets (equivalent with 20,496 lines. The pss10010 contains 16 seed pools and pss10021, 14 seed pools. Others contain 20 seed pools.)
Background ecotype: Columbia
Vector for transformation with Agrobacterium: pBIG2113SF(pBI121 derivative)
Selection marker: hygromycin
Specific feature: Provided as seed pools for screening. Arabidopsis full-length cDNA (RAFL) was introduced with 35S promoter to produce dominant phenotype.
Specific conditions for distribution:
  1. When the RECIPIENT has filed a patent application on the invention conceived or made during the course of research studies directly using the BIOLOGICAL RESOURCE, the RECIPIENT agrees to promptly notify RIKEN Plant Science Center upon filing the application with specifications.
  2. When the RECIPIENT publishes his/her research, acknowledge RIKEN PSC and refer designated manuscripts shown below.
    (For the detailed information, please email to plant@brc.riken.jp )
Reference:
Ichikawa T, Nakazawa M, Kawashima M, Iizumi H, Kuroda H, Kondou Y, Tuhara Y, Suzuki K, Ishikawa A, Seki M, Fujita M, Motohashi R, Nagata N, Takagi T, Shinozaki K and Matsui M (2006) Plant J. 48:974-985 PMID: 17227551
Requirements for the safe handling, storage, transport and use:
  1. This BIOLOGICAL MATERIAL is the seed of Arabidopsis thaliana.
  2. This BIOLOGICAL MATERIAL harbor HPT gene (from E.coli), promoter of CaMV 35S RNA gene (from cauliflower mosaic virus), LB, RB and NOS-terminator sequences of T-DNA (Agrobacterium tumefaciens).
  3. This BIOLOGICAL MATERIAL is for laboratory use (contained use) only. Do not use it for “Intentional introduction into the environment”.
This BIOLOGICAL MATERIAL and its progeny should be inactivated by autoclave or equivalent procedure before disposed as a waste.