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Resource List

Arabidopsis thaliana Arabidopsis thaliana
Brachypodium Brachypodium distachyon
populus Populus nigra
oryza Oryza sativa
lotus Lotus japonicus
lycopersicom Lycopersicon esculentum Mill
striga Striga hermonthica
nicotiana Nicotiana tabacum
(BY-2 cell)
brassica Brassica rapa
thellungiella Thellungiella halophila
physcomitrella Physcomitrella patens
manihot Manihot esculenta Crantz

Embryogenic callus (domestic only)

Species name: Embryogenic callus of Brachypodium distachyon

Resource Number: rpc10001

Species: Brachypodium distachyon

Common name:

Line name: Bd21

Characteristic feature: Able to use for transformation using Agrobacterium / particle bombardment

Medium: Callus induction medium 2

Method for maintenance: Transfer a portion (approx. 2mm in diameter) of growing callus to a fresh medium every 10 days. The growth of callus may be much slower under lower temperature such as 25℃. If it is the case, you would better wait for 14 days untill next subculture.

Temperature condition: 27℃ (Lower limit: 25℃)

Light condition: Dark

Reference: Nat. Protoc. 4, 638-649 (2009)

Specific condition in MTA: none

Depositor: n/a

Developer: n/a

Note: We recommend recipients to use the callus for transformation within one month after its arrival.