{"id":328,"date":"2007-01-29T15:39:24","date_gmt":"2007-01-29T06:39:24","guid":{"rendered":"http:\/\/vps36.rbrc.jp:8179\/?p=328"},"modified":"2014-12-04T16:46:41","modified_gmt":"2014-12-04T07:46:41","slug":"%e3%83%88%e3%83%a9%e3%83%b3%e3%82%b9%e3%83%9d%e3%82%be%e3%83%b3%e3%82%bf%e3%82%b0%e3%83%a9%e3%82%a4%e3%83%b3%e3%83%bb%e3%83%9b%e3%83%a2%e7%a8%ae%e5%ad%90%e3%81%ae%e6%8f%90%e4%be%9b%e3%81%ab%e3%81%a4-2","status":"publish","type":"post","link":"http:\/\/epd.brc.riken.jp\/ja\/archives\/328","title":{"rendered":"\u30c8\u30e9\u30f3\u30b9\u30dd\u30be\u30f3\u30bf\u30b0\u30e9\u30a4\u30f3\u30fb\u30db\u30e2\u7a2e\u5b50\u306e\u63d0\u4f9b\u306b\u3064\u3044\u3066"},"content":{"rendered":"<p>\u7406\u7814GSC \u304c\u958b\u767a\u3057\u305f\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u30c8\u30e9\u30f3\u30b9\u30dd\u30be\u30f3\u30bf\u30b0\u30e9\u30a4\u30f3\u306e\u3046\u3061\u907a\u4f1d\u5b50\u5185\u90e8\u307e\u305f\u306f\u8fd1\u508d\u306b Ds element \u304c\u633f\u5165\u3055\u308c\u305f 926\u7cfb\u7d71 \u306b\u3064\u3044\u3066<a href=\"http:\/\/www.brc.riken.jp\/lab\/epd\/news\/070123ex.shtml\" target=\"_blank\">\u30db\u30e2\u7a2e\u5b50<\/a>\u306e\u63d0\u4f9b\u3092\u958b\u59cb\u3057\u307e\u3059\u3002\u3054\u95a2\u5fc3\u304c\u3042\u308b\u65b9\u306f\u4ee5\u4e0b\u306e\u8aac\u660e\u3092\u304a\u8aad\u307f\u3044\u305f\u3060\u3044\u305f\u3046\u3048\u3067\u7533\u8fbc\u307f\u3092\u304a\u9858\u3044\u81f4\u3057\u307e\u3059\u3002<\/p>\n<ol>\n<li>\u307e\u305a\u3054\u95a2\u5fc3\u306e\u3042\u308b\u7cfb\u7d71\u306b\u3064\u3044\u3066\u30db\u30e2\u7a2e\u5b50\u304c\u5229\u7528\u53ef\u80fd\u304b\u3069\u3046\u304b\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u306e<a href=\"http:\/\/www.brc.riken.jp\/lab\/epd\/catalog\/transposon.html\" target=\"_blank\">\u30aa\u30f3\u30e9\u30a4\u30f3\u30ab\u30bf\u30ed\u30b0<\/a>\u3092\u30ad\u30fc\u30ef\u30fc\u30c9\u691c\u7d22\u3057\u305f\u969b\u306b\u8868\u793a\u3055\u308c\u308b\u691c\u7d22\u7d50\u679c\u753b\u9762\u3067\u30db\u30e2\u7a2e\u5b50\u304c\u5229\u7528\u53ef\u80fd\u306a\u7cfb\u7d71\u306b\u306f\u25cb\u5370\u304c\u4ed8\u4e0e\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u30db\u30e2\u7a2e\u5b50\u304c\u5229\u7528\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3067\u304d\u63d0\u4f9b\u3092\u7533\u3057\u8fbc\u307e\u308c\u308b\u5834\u5408\u306b\u306f\u3001\u63d0\u4f9b\u7533\u8fbc\u66f8\u306e\u30ea\u30bd\u30fc\u30b9\u756a\u53f7\u306e\u6b04\u306b psh \u3092\u3054\u8a18\u5165\u304f\u3060\u3055\u3044\u3002\u4f8b\u3048\u3070 pst99999 \u306e\u30db\u30e2\u7a2e\u5b50\u3092\u3054\u8acb\u6c42\u3055\u308c\u308b\u969b\u306b\u306f psh99999 \u3068\u3054\u8a18\u5165\u304a\u9858\u3044\u3057\u307e\u3059\u3002<\/li>\n<li>\u5e73\u6210\uff11\uff16\u5e74\u5ea6\u4ee5\u964d\u306b\u30c8\u30e9\u30f3\u30b9\u30dd\u30be\u30f3\u30bf\u30b0\u30e9\u30a4\u30f3\u306e\u63d0\u4f9b\u540c\u610f\u66f8\u3092\u7de0\u7d50\u3055\u308c\u3066\u3044\u308b\u5834\u5408\u306b\u306f\u3001\u30db\u30e2\u7a2e\u5b50\u306e\u3054\u8acb\u6c42\u306e\u969b\u306b\u6539\u3081\u3066 MTA \u3092\u7de0\u7d50\u3059\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/li>\n<li>\u30db\u30e2\u7a2e\u5b50\u306f \uff11\u7cfb\u7d71 \u3042\u305f\u308a 500\u7c92 \u3092\u5358\u4f4d\u3068\u3057\u3066\u63d0\u4f9b\u81f4\u3057\u307e\u3059\u3002<\/li>\n<li>\u63d0\u4f9b\u624b\u6570\u6599\u306f\uff11\u7cfb\u7d71\u3042\u305f\u308a 10\u500b\u5206 \u3068\u3057\u3066<a href=\"http:\/\/www.brc.riken.jp\/lab\/epd\/fee\/fee.shtml\" target=\"_blank\">\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u63b2\u8f09\u306e\u8868<\/a>\u306b\u3042\u3066\u306f\u3081\u3066\u8a08\u7b97\u3057\u307e\u3059\u3002\u300c\u5927\u91cf\u30e6\u30fc\u30b6\u30fc\u300d\u3068\u3057\u3066\u304a\u652f\u6255\u3044\u3044\u305f\u3060\u304f\u5834\u5408\u306b\u306f10\u7cfb\u7d71\u307e\u30679,135\u5186\uff08\u5b66\u8853\u7814\u7a76\u6a5f\u95a2\uff09\u3068\u306a\u308a\u307e\u3059\u3002\u306a\u304a\u300c\u5c11\u91cf\u30e6\u30fc\u30b6\u30fc\u300d\u3068\u3057\u3066\u65e2\u306b\u304a\u652f\u6255\u3044\u3092\u3044\u305f\u3060\u3044\u3066\u3044\u308b\u5834\u5408\u306b\u306f\u3001\u30db\u30e2\u7a2e\u5b50 \uff11\u7cfb\u7d71 \u306b\u3064\u304d \uff11\u56de\u5206 \u306e\u8acb\u6c42\u6a29\u3092\u4f7f\u7528\u3055\u308c\u305f\u3068\u307f\u306a\u3057\u307e\u3059\u3002\u4f8b\u3048\u3070\u30db\u30e2\u7a2e\u5b50 \uff12\u7cfb\u7d71 \u3092\u3054\u8acb\u6c42\u3055\u308c\u305f\u5834\u5408\u306b\u306f 10\u56de \u306e\u8acb\u6c42\u6a29\u306e\u3046\u3061 \uff12\u56de \u3092\u4f7f\u7528\u3055\u308c\u305f\u3068\u3057\u307e\u3059\u3002<\/li>\n<li>\u30db\u30e2\u7a2e\u5b50\u306e\u3054\u8acb\u6c42 \uff11\u4ef6 \u306b\u3064\u304d Nossen \u306e\u91ce\u751f\u578b\u682a 500\u7c92 \u3092 control \u7528\u3068\u3057\u3066\u540c\u68b1\u3057\u307e\u3059\u3002\u306a\u304a\u8868\u73fe\u578b\u306e\u6bd4\u8f03\u3092\u53b3\u5bc6\u306b\u884c\u306a\u3046\u5834\u5408\u306b\u306f Ds element \u53ca\u3073 Ac element \u304c\u305d\u308c\u305e\u308c\u633f\u5165\u3055\u308c\u3066\u3044\u308b<a href=\"\/en\/seed\/transp\/transplist\" target=\"_blank\">\u89aa\u682a<\/a>\u3082\u3042\u308f\u305b\u3066\u304a\u4f7f\u3044\u3044\u305f\u3060\u304f\u3053\u3068\u3092\u304a\u52e7\u3081\u81f4\u3057\u307e\u3059\u3002<\/li>\n<li>\u7406\u7814BRC \u3067\u306f\u63d0\u4f9b\u81f4\u3057\u307e\u3059\u7a2e\u5b50\u30b9\u30c8\u30c3\u30af\u3092 PCR \u306b\u3088\u308a\u89e3\u6790\u3057\u3001homozygous \u306e\u7834\u58ca\u682a\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304a\u308a\u307e\u3059\u304c\u3001\u4e07\u304c\u4e00\u306b\u3082\u30d8\u30c6\u30ed\u306e\u7a2e\u5b50\u304c\u6df7\u5165\u3057\u3066\u304a\u308a\u307e\u3057\u305f\u5834\u5408\u306b\u306f\u304a\u77e5\u3089\u305b\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u30c8\u30e9\u30f3\u30b9\u30dd\u30be\u30f3\u30bf\u30b0\u30e9\u30a4\u30f3\u306e\u63d0\u4f9b\u3092\u53d7\u3051\u308b\u306b\u306f\u4e88\u3081<a href=\"http:\/\/www.brc.riken.jp\/lab\/epd\/cartagena\/cartagena.shtml\" target=\"_blank\">\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u751f\u7269\u306e\u53d6\u6271\u3044<\/a>\u306b\u95a2\u3057\u3066\u3054\u6240\u5c5e\u306e\u6a5f\u95a2\u3088\u308a\u9069\u5207\u306a\u5185\u5bb9\u3067\u306e\u8a31\u53ef\u3092\u53d7\u3051\u3066\u3044\u308b\u3053\u3068\u304c\u5fc5\u8981\u3067\u3059\u3002<\/li>\n<li>\u633f\u5165\u5909\u7570\u4f53\u3092\u304a\u4f7f\u3044\u306b\u306a\u308b\u6642\u306f\u633f\u5165\u4f4d\u7f6e\u4ee5\u5916\u306b\u5909\u7570\u304c\u751f\u3058\u3066\u3044\u308b\u53ef\u80fd\u6027\u3082\u8003\u616e\u3057\u3066\u5b9f\u9a13\u8a08\u753b\u3092\u305f\u3066\u3089\u308c\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u81f4\u3057\u307e\u3059\u3002<\/li>\n<li>\u30c8\u30e9\u30f3\u30b9\u30dd\u30be\u30f3\u30bf\u30b0\u30e9\u30a4\u30f3\u306b\u95a2\u3057\u3066\u306e\u8a73\u7d30\u60c5\u5831\u306f<a href=\"http:\/\/www.brc.riken.jp\/lab\/epd\/catalog\/transp.shtml\" target=\"_blank\">\u3053\u3061\u3089<\/a>\u304b\u3089\u95b2\u89a7\u3067\u304d\u307e\u3059\u3002<\/li>\n<li>\u5f15\u304d\u7d9a\u304d\u907a\u4f1d\u5b50\u5185\u90e8\u307e\u305f\u306f\u3054\u304f\u8fd1\u508d\u306b Ds \u304c\u633f\u5165\u3055\u308c\u305f\u7cfb\u7d71\u306b\u3064\u3044\u3066\u9806\u6b21\u30db\u30e2\u7a2e\u5b50\u306e\u6e96\u5099\u3092\u9032\u3081\u3066\u3044\u304d\u307e\u3059\u3002\u6574\u5099\u72b6\u6cc1\u306b\u3064\u3044\u3066\u306f HP \u7b49\u3088\u308a\u304a\u77e5\u3089\u305b\u81f4\u3057\u307e\u3059\u3002<\/li>\n<\/ol>\n<p align=\"right\">(2007\/1\/29)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7406\u7814GSC \u304c\u958b\u767a\u3057\u305f\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u30c8\u30e9\u30f3\u30b9\u30dd\u30be\u30f3\u30bf\u30b0\u30e9\u30a4\u30f3\u306e\u3046\u3061\u907a\u4f1d\u5b50\u5185\u90e8\u307e\u305f\u306f\u8fd1\u508d\u306b Ds element \u304c\u633f\u5165\u3055\u308c\u305f 926\u7cfb\u7d71 \u306b\u3064\u3044\u3066\u30db\u30e2\u7a2e\u5b50\u306e\u63d0\u4f9b\u3092\u958b\u59cb\u3057\u307e\u3059\u3002\u3054\u95a2\u5fc3\u304c\u3042\u308b\u65b9\u306f\u4ee5\u4e0b\u306e\u8aac\u660e\u3092\u304a\u8aad\u307f\u3044\u305f\u3060\u3044\u305f\u3046\u3048\u3067\u7533\u8fbc\u307f\u3092\u304a\u9858\u3044\u81f4\u3057\u307e\u3059\u3002 \u307e\u305a\u3054\u95a2\u5fc3\u306e\u3042\u308b\u7cfb\u7d71\u306b\u3064\u3044\u3066\u30db\u30e2\u7a2e\u5b50\u304c\u5229\u7528\u53ef\u80fd\u304b\u3069\u3046\u304b\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u306e\u30aa\u30f3\u30e9\u30a4\u30f3\u30ab\u30bf\u30ed\u30b0\u3092\u30ad\u30fc\u30ef\u30fc\u30c9\u691c\u7d22\u3057\u305f\u969b\u306b\u8868\u793a\u3055\u308c\u308b\u691c\u7d22\u7d50\u679c\u753b\u9762 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":"","_wp_rev_ctl_limit":""},"categories":[11],"tags":[],"class_list":["post-328","post","type-post","status-publish","format-standard","hentry","category-news"],"_links":{"self":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/posts\/328","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=328"}],"version-history":[{"count":4,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/posts\/328\/revisions"}],"predecessor-version":[{"id":2925,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/posts\/328\/revisions\/2925"}],"wp:attachment":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=328"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/categories?post=328"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/tags?post=328"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}