{"id":1733,"date":"2013-06-06T15:03:18","date_gmt":"2013-06-06T06:03:18","guid":{"rendered":"http:\/\/epd.brc.riken.jp:7311\/?page_id=1733"},"modified":"2013-07-09T14:27:38","modified_gmt":"2013-07-09T05:27:38","slug":"qa_nazuna6","status":"publish","type":"page","link":"http:\/\/epd.brc.riken.jp\/ja\/manual\/nazuna\/qa_nazuna6","title":{"rendered":"\u57f9\u990a\u7d30\u80de\u3092\u7528\u3044\u305ftransient assay\u7cfb\u3067\u306e\u86cb\u767d\u767a\u73fe\u306e\u78ba\u8a8d\u306b\u3064\u3044\u3066"},"content":{"rendered":"<p><a name=\"head\"><\/a><\/p>\n<p align=\"right\">\u2192 <a href=\"\/ja\/wp-content\/uploads\/data\/nazuna6.rtf\">&gt;\u8a18\u4e8b\u306e\u4e00\u62ec\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\uff08 Word \u6587\u66f8\uff09<\/a><\/p>\n<p><strong>\u57f9\u990a\u7d30\u80de\u3084\u30d7\u30ed\u30c8\u30d7\u30e9\u30b9\u30c8\u3092\u5229\u7528\u3057\u305f transient assay \u7cfb\u3067\u86cb\u767d\u306e\u767a\u73fe\u3092\u78ba\u8a8d\u3059\u308b\u65b9\u6cd5\u306b\u3064\u3044\u3066\u306e\u60c5\u5831\u3067\u3059\u3002<\/strong><\/p>\n<p>\u3010Thu, 27 Feb 2003 21:39:57\u3011<br \/>\n[nazuna 01835] Transient assay \u306e\u969b\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u91cf&#8212;&#8212;&#8212;&#8212;&#8212;<\/p>\n<p style=\"padding-left: 30px;\">\u79c1\u305f\u3061\u306e\u3068\u3053\u308d\u3067\u306f\u30a4\u30cd Oc \u7d30\u80de\u306e\u30d7\u30ed\u30c8\u30d7\u30e9\u30b9\u30c8\u3067\u30c8\u30e9\u30f3\u30b8\u30a8\u30f3\u30c8\u306b\u767a\u73fe\u3055\u305b\u305f\u30a8\u30d4\u30c8\u30fc\u30d7\u30bf\u30b0\u4ed8\u304d\u306e\u8ee2\u5199\u56e0\u5b50\u3084\u30d7\u30ed\u30c6\u30a4\u30f3\u30ad\u30ca\u30fc\u30bc\u3092\u30a6\u30a7\u30b9\u30bf\u30f3\u3067\u691c\u51fa\u3057\u3066\u3044\u307e\u3059\u3002\u304b\u306a\u308a\u5f37\u3044\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u4f7f\u3063\u3066\u3044\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u304c\u3001\u7d50\u69cb\u697d\u306b\u691c\u51fa\u53ef\u80fd\u3067\u3059\u3002\u6297\u4f53\u306e\u5584\u3057\u60aa\u3057\u306b\u3082\u4f9d\u5b58\u3059\u308b\u3068\u601d\u3044\u307e\u3059\u304c\u3001\u5e02\u8ca9\u306e\u6297 HA \u6297\u4f53\u3092\u4f7f\u3046\u9650\u308a\u306f\u3046\u307e\u304f\u3044\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<p><a href=\"#head\"><img loading=\"lazy\" decoding=\"async\" title=\"arrow_head\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow_head.gif\" width=\"16\" height=\"16\" \/><\/a> \u3010Fri, 28 Feb 2003 09:38:59\u3011<br \/>\n[nazuna 01837] Transient assay \u306e\u969b\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u91cf&#8212;&#8212;&#8212;&#8212;&#8212;<\/p>\n<p style=\"padding-left: 30px;\">\u3046\u3061\u306e\u30e9\u30dc\u3067\u306f\u3001\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u306e\u57f9\u990a\u7d30\u80de\u306b PEG \u6cd5\u3067\u300135S \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3092\u7528\u3044\u3066 myc-epitope \u3092\u3064\u3051\u305f\u3001SNARE \u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30c8\u30e9\u30f3\u30b8\u30a7\u30f3\u30c8\u3067\u767a\u73fe\u3055\u305b\u3066\u3001myc \u6297\u4f53\u3092 \u7528\u3044\u3066\u30a6\u30a7\u30b9\u30bf\u30f3\u3092\u884c\u3063\u3066\u3044\u307e\u3059\u3002ECL \u3067\u691c\u51fa\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u767a\u73fe\u6761\u4ef6\u304c\u3044\u3044\u5834\u5408\u306b\u306f\u3001\u5bb9\u6613\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u76ee\u7684\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u91cf\u306b\u3082\u3088\u308b\u3068\u601d\u3044\u307e\u3059\u304c\u3001\u3088\u3044\u6297\u4f53\u3092\u4f7f\u3048\u3070\u3001\u767a\u73fe\u3092\u30c8\u30e9\u30f3\u30b8\u30a7\u30f3\u30c8\u3067\u89e3\u6790\u3059\u308b\u306e\u306f\u305d\u3093\u306a\u306b\u96e3\u3057\u3044\u3053\u3068\u3067\u306f\u306a\u3044\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n<p><a href=\"#head\"><img loading=\"lazy\" decoding=\"async\" title=\"arrow_head\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow_head.gif\" width=\"16\" height=\"16\" \/><\/a> \u3010Tue, 04 Mar 2003 13:57:00\u3011<br \/>\n[nazuna 01839] Transient assay \u306e\u969b\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u91cf&#8212;&#8212;&#8212;&#8212;&#8212;<\/p>\n<p style=\"padding-left: 30px;\">\u30a6\u30c1\u306e\u7814\u7a76\u5ba4\u3067\u306f myc \u6297\u4f53\u3068 T7 \u6297\u4f53\u3092\u5229\u7528\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u554f\u984c\u306a\u304f\u30a6\u30a8\u30b9\u30bf\u30f3\u3067\u691c\u51fa\u3067\u304d\u3066\u307e\u3059\u3088\u3002\u57f9\u990a\u7d30\u80de\u306f\u30a2\u30e9\u30d3\u3067\u3059\u3002\u5f62\u8cea\u8ee2\u63db\u52b9\u7387\u306f 10-50% \u304f\u3089\u3044\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n<p style=\"padding-left: 30px;\">\u79c1\u3082\u30bf\u30d0\u30b3 BY-2 \u7d30\u80de\u3001\u30ca\u30ba\u30ca\u57f9\u990a\u7d30\u80de\u7531\u6765\u306e\u30d7\u30ed\u30c8\u30d7\u30e9\u30b9\u30c8\u3067\u767a\u73fe\u3055\u305b\u305f\u30bf\u30f3\u30d1\u30af\u3092\u30a6\u30a8\u30b9\u30bf\u30f3\u3067\u78ba\u8a8d\u3057\u307e\u3057\u305f\u304c\u3001\u554f\u984c\u306a\u304f\u691c\u51fa\u3067\u304d\u307e\u3057\u305f\u3002\u304b\u306a\u308a\u767a\u73fe\u306e\u5f31\u3044 own promoter \u3092\u767a\u73fe\u306b\u7528\u3044\u305f\u306e\u3067\u691c\u51fa\u3067\u304d\u308b\u304b\u7591\u308f\u3057\u304b\u3063\u305f\u306e\u3067\u3059\u304c\u3001\u79c1\u3069\u3082\u304c\u4f7f\u3063\u3066\u3044\u308b\u7cfb\u3067\u306f\u5341\u5206\u306a\u767a\u73fe\u304c\u5f97\u3089\u308c\u305f\u3088\u3046\u3067\u3059\u3002\u5927\u5207\u306a\u306e\u306f\u6297\u4f53\u306e\u529b\u4fa1\u3067\u3042\u308b\u3068\u601d\u308f\u308c\u307e\u3059\u3002\u79c1\u304c\u7528\u3044\u305f\u306e\u306f\u5e02\u8ca9\u306e c-Myc \u6297\u4f53\u306a\u306e\u3067\u3001\u529b\u4fa1\u306e\u3082\u3063\u3068\u4f4e\u3044\u3082\u306e\u3092\u7528\u3044\u305f\u5834\u5408\u306f\u554f\u984c\u304c\u751f\u3058\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n<p><a href=\"#head\"><img loading=\"lazy\" decoding=\"async\" title=\"arrow_head\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/arrow_head.gif\" width=\"16\" height=\"16\" \/> Go to Head<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u2192 &gt;\u8a18\u4e8b\u306e\u4e00\u62ec\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\uff08 Word \u6587\u66f8\uff09 \u57f9\u990a\u7d30\u80de\u3084\u30d7\u30ed\u30c8\u30d7\u30e9\u30b9\u30c8\u3092\u5229\u7528\u3057\u305f transient assay \u7cfb\u3067\u86cb\u767d\u306e\u767a\u73fe\u3092\u78ba\u8a8d\u3059\u308b\u65b9\u6cd5\u306b\u3064\u3044\u3066\u306e\u60c5\u5831\u3067\u3059\u3002 \u3010Thu, 27 Feb 2003 21:39:57\u3011 [nazuna 01835] Transient assay \u306e\u969b\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u91cf&#8212;&#8212;&#8212;&#8212;&#8212; \u79c1\u305f\u3061\u306e\u3068\u3053\u308d [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1597,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-1733","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/1733","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=1733"}],"version-history":[{"count":4,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/1733\/revisions"}],"predecessor-version":[{"id":1945,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/1733\/revisions\/1945"}],"up":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/1597"}],"wp:attachment":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=1733"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}