{"id":1622,"date":"2013-05-30T14:55:23","date_gmt":"2013-05-30T05:55:23","guid":{"rendered":"http:\/\/epd.brc.riken.jp:7311\/?page_id=1622"},"modified":"2021-07-28T13:47:23","modified_gmt":"2021-07-28T04:47:23","slug":"cryopreservation_0714","status":"publish","type":"page","link":"http:\/\/epd.brc.riken.jp\/ja\/manual\/cryopreservation_0714","title":{"rendered":"\u690d\u7269\u57f9\u990a\u7d30\u80de\u306e\u8d85\u4f4e\u6e29\u4fdd\u5b58\u6280\u8853"},"content":{"rendered":"<p>\u690d\u7269\u57f9\u990a\u7d30\u80de\u306f\u52d5\u7269\u7d30\u80de\u3068\u7570\u306a\u308a\u6db2\u4f53\u7a92\u7d20\u4e2d\u3067\u306e\u4fdd\u5b58\u304c\u96e3\u3057\u304f\u3001\u751f\u7d30\u80de\u3067\u7dad\u6301\u3057\u7d9a\u3051\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u751f\u7d30\u80de\u3067\u306e\u7dad\u6301\u306f\u3001\u7d99\u4ee3\u4f5c\u696d\u306e\u30df\u30b9\u3084\u707d\u5bb3\u306b\u3088\u308b\u505c\u96fb\u7b49\u3067\u306e\u30ea\u30bd\u30fc\u30b9\u306e\u6ec5\u5931\u306e\u5371\u967a\u3001\u66f4\u306b\u306f\u30ea\u30bd\u30fc\u30b9\u306e\u7279\u6027\u306e\u5909\u5316\u7b49\u306e\u5371\u967a\u6027\u3092\u4f34\u3044\u307e\u3059\u3002\u305d\u3053\u3067\u5b9f\u9a13\u690d\u7269\u958b\u767a\u5ba4\u3067\u306f\u3001\u7c21\u4fbf\u306a\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u3088\u308b\u57f9\u990a\u7d30\u80de\u306e\u8d85\u4f4e\u6e29\u4fdd\u5b58\u6280\u8853\u3092\u958b\u767a\u3057\u3001\u4e8b\u696d\u306b\u9069\u7528\u3057\u3066\u304a\u308a\u307e\u3059\u3002\u307e\u305f\u5b98\u6c11\u306e\u7814\u7a76\u8005\u3092\u5bfe\u8c61\u3068\u3057\u305f\u6280\u8853\u7814\u4fee\u4e8b\u696d\u306b\u304a\u3044\u3066\u306f\u3001\u5e73\u6210 16 \u5e74\u5ea6\u3088\u308a\u4ee3\u8868\u7684\u306a\u7cfb\u7d71\u3067\u3042\u308b\u30bf\u30d0\u30b3 BY-2 \u7d30\u80de\u306e\u8d85\u4f4e\u6e29\u4fdd\u5b58\u6280\u8853\u306b\u3064\u3044\u3066\u3001\u958b\u767a\u8005\u306e\u5c0f\u6797\u4fca\u5f18\u5c02\u4efb\u7814\u7a76\u54e1\u304c\u8b1b\u5e2b\u3068\u306a\u308a\u7814\u4fee\u3092\u958b\u50ac\u3057\u3066\u304a\u308a\u307e\u3059\u3002\u3054\u95a2\u5fc3\u306e\u3042\u308b\u65b9\u306f\u3001\u4e0b\u8a18\u30ea\u30f3\u30af\u3088\u308a\u6280\u8853\u7814\u4fee\u306e\u8cc7\u6599\u3092\u95b2\u89a7\u3067\u304d\u307e\u3059\u306e\u3067\u3054\u6d3b\u7528\u304f\u3060\u3055\u3044\u3002\u3054\u8cea\u554f\u7b49\u306f<a href=\"mailto:plant.brc@riken.jp\">plant.brc@riken.jp<\/a>\u00a0\u307e\u3067\u304a\u9858\u3044\u81f4\u3057\u307e\u3059\u3002<\/p>\n<p>\u25ce\u53c2\u8003\u6587\u732e<\/p>\n<p style=\"padding-left: 30px;\"><a href=\"https:\/\/www.jstage.jst.go.jp\/article\/plantbiotechnology\/22\/2\/22_2_105\/_pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Kobayashi T, Niino T, Kobayashi M (2005) Simple cryopreservation protocol with an encapsulation technique for tobacco BY-2 suspension cell cultures. Plant Biotechnology 22: 105-112<img decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/pdficon_s.png\" border=\"0\" \/><\/a><\/p>\n<p style=\"padding-left: 30px;\"><a href=\"https:\/\/www.jstage.jst.go.jp\/article\/plantbiotechnology\/23\/3\/23_3_333\/_pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Kobayashi T, Niino T, Kobayashi M (2006) Cryopreservation of tobacco BY-2 suspension cell cultures by vitrification with encapsulation. Plant Biotechnology 24: 333-338<img decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/pdficon_s.png\" border=\"0\" \/><\/a><\/p>\n<p>\u25ce\u7814\u4fee\u8cc7\u6599\u306e\u95b2\u89a7<\/p>\n<p style=\"padding-left: 30px;\">\u00b7\u00a0<a href=\"\/ja\/wp-content\/uploads\/data\/protocol.pdf\">\u30d7\u30ed\u30c8\u30b3\u30eb<img decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/pdficon_s.png\" border=\"0\" \/><\/a><\/p>\n<p style=\"padding-left: 30px;\">\u00b7\u00a0<a href=\"\/ja\/wp-content\/uploads\/data\/lecture.pdf\">\u5b9f\u7fd2\u8cc7\u6599<img decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/pdficon_s.png\" border=\"0\" \/><\/a><\/p>\n<p style=\"padding-left: 30px;\">\u00b7\u00a0<a href=\"\/ja\/wp-content\/uploads\/data\/documents.pdf\">\u7814\u4fee\u8b1b\u7fa9\u8cc7\u6599<img decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/pdficon_s.png\" border=\"0\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u690d\u7269\u57f9\u990a\u7d30\u80de\u306f\u52d5\u7269\u7d30\u80de\u3068\u7570\u306a\u308a\u6db2\u4f53\u7a92\u7d20\u4e2d\u3067\u306e\u4fdd\u5b58\u304c\u96e3\u3057\u304f\u3001\u751f\u7d30\u80de\u3067\u7dad\u6301\u3057\u7d9a\u3051\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u751f\u7d30\u80de\u3067\u306e\u7dad\u6301\u306f\u3001\u7d99\u4ee3\u4f5c\u696d\u306e\u30df\u30b9\u3084\u707d\u5bb3\u306b\u3088\u308b\u505c\u96fb\u7b49\u3067\u306e\u30ea\u30bd\u30fc\u30b9\u306e\u6ec5\u5931\u306e\u5371\u967a\u3001\u66f4\u306b\u306f\u30ea\u30bd\u30fc\u30b9\u306e\u7279\u6027\u306e\u5909\u5316\u7b49\u306e\u5371\u967a\u6027\u3092\u4f34\u3044\u307e\u3059\u3002\u305d\u3053\u3067\u5b9f\u9a13\u690d\u7269\u958b\u767a\u5ba4\u3067\u306f\u3001\u7c21\u4fbf\u306a\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u3088\u308b\u57f9\u990a\u7d30\u80de\u306e\u8d85\u4f4e\u6e29\u4fdd\u5b58\u6280\u8853\u3092\u958b\u767a\u3057\u3001\u4e8b\u696d\u306b\u9069\u7528\u3057\u3066\u304a\u308a\u307e\u3059\u3002\u307e\u305f\u5b98\u6c11\u306e\u7814\u7a76\u8005\u3092\u5bfe\u8c61\u3068\u3057\u305f\u6280\u8853\u7814\u4fee\u4e8b\u696d\u306b\u304a\u3044\u3066\u306f\u3001\u5e73\u6210 16 \u5e74\u5ea6\u3088\u308a\u4ee3 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":1590,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-1622","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/1622","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/comments?post=1622"}],"version-history":[{"count":10,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/1622\/revisions"}],"predecessor-version":[{"id":5133,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/1622\/revisions\/5133"}],"up":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/pages\/1590"}],"wp:attachment":[{"href":"http:\/\/epd.brc.riken.jp\/ja\/wp-json\/wp\/v2\/media?parent=1622"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}