{"id":1611,"date":"2013-05-30T14:50:04","date_gmt":"2013-05-30T05:50:04","guid":{"rendered":"http:\/\/epd.brc.riken.jp:7311\/?page_id=1611"},"modified":"2026-04-24T14:05:36","modified_gmt":"2026-04-24T05:05:36","slug":"rule","status":"publish","type":"page","link":"http:\/\/epd.brc.riken.jp\/ja\/order_top\/rule","title":{"rendered":"\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u751f\u7269\u306e\u904b\u642c\u306b\u95a2\u308f\u308b\u898f\u5247"},"content":{"rendered":"

\u30bf\u30b0\u30e9\u30a4\u30f3\u7b49\u3001\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u7a2e\u5b50\u306e\u4fdd\u7ba1\u3001\u904b\u642c\u53ca\u3073\u4f7f\u7528\u306f\u6cd5\u5f8b\u306b\u57fa\u3065\u3044\u305f\u898f\u5236<\/a>\u306e\u5bfe\u8c61\u3068\u306a\u3063\u3066\u304a\u308a\u307e\u3059\u3002\u7406\u7814BRC\u3067\u306f\u3001\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u751f\u7269\u3092\u3054\u63d0\u4f9b\u3059\u308b\u969b\u306b\u3001\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u5b9f\u9a13\u306e\u8a08\u753b\u66f8\u4e26\u3073\u306b\u627f\u8a8d\u66f8\u306e\u5199\u3057*\u3001\u3042\u308b\u3044\u306f\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u751f\u7269\u306e\u63d0\u4f9b\u78ba\u8a8d\u66f8**\u3092\u304a\u9001\u308a\u3044\u305f\u3060\u304d\u3001\u30ea\u30bd\u30fc\u30b9\u306e\u4f7f\u7528\u304c\u6cd5\u5f8b\u306b\u6cbf\u3063\u3066\u884c\u308f\u308c\u308b\u3053\u3068\u3092\u5229\u7528\u8005\u3088\u308a\u901a\u77e5\u3044\u305f\u3060\u3044\u3066\u304a\u308a\u307e\u3059\u3002\u3054\u5354\u529b\u3092\u304a\u9858\u3044\u81f4\u3057\u307e\u3059\u3002<\/p>\n

*\u5229\u7528\u8005\u304c\u6240\u5c5e\u3059\u308b\u6a5f\u95a2\u306b\u304a\u3044\u3066\u8a72\u5f53\u3059\u308b\u66f8\u985e\u304c\u6574\u5099\u3055\u308c\u3066\u3044\u306a\u3044\u5834\u5408\u306b\u306f\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002( plant.brc@riken.jp<\/a> )
\n**\u00b7 \u63d0\u4f9b\u78ba\u8a8d\u66f8<\/strong>\u3000(MS Word)<\/a>
\n    \u00b7 \u63d0\u4f9b\u78ba\u8a8d\u66f8\u306e\u8a18\u5165\u4f8b<\/strong>\u3000(MS Word)<\/a><\/p>\n

\u306a\u304a\u3001\u5229\u7528\u8005\u304c\u6240\u5c5e\u3059\u308b\u6a5f\u95a2\u3078\u306e\u5c4a\u3051\u51fa\u53ca\u3073\u5b9f\u9a13\u8a08\u753b\u66f8\u306e\u5909\u66f4\u306b\u3064\u3044\u3066\u306f\u5229\u7528\u8005\u306e\u8cac\u4efb\u306b\u304a\u3044\u3066\u884c\u3063\u3066\u4e0b\u3055\u3044\u3002\u5f53\u5ba4\u3088\u308a\u63d0\u4f9b\u3059\u308b\u30bf\u30b0\u30e9\u30a4\u30f3\u306e\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u5b9f\u9a13\u306b\u95a2\u308f\u308b\u60c5\u5831\u306f\u4ee5\u4e0b\u306e\u3068\u304a\u308a\u3067\u3059\u3002<\/p>\n

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<\u30c8\u30e9\u30f3\u30b9\u30dd\u30be\u30f3\u30bf\u30b0\u30e9\u30a4\u30f3\u306e\u60c5\u5831\u306b\u3064\u3044\u3066><\/strong><\/p>\n

\u4f5c\u6210\u306b\u7528\u3044\u305f\u30d9\u30af\u30bf\u30fc\u540d\uff1a<\/strong>
\nT-DNA \u30d9\u30af\u30bf\u30fc\uff08pCGN\u7cfb\uff09\uff08Fedoroff et al. Plant Journal 3, 273-289 (1993))
\n\u5bbf\u4e3b\u306b\u7d44\u307f\u8fbc\u307e\u308c\u305f\u907a\u4f1d\u5b50\u53ca\u3073\u7531\u6765\uff1a<\/strong>
\nT-DNA region {NPTII, HPT and GUS genes (from E. coli), ALS gene (from A. thaliana), Ds element (from Zea mays), CaMV 35S promoter and CaMV 19S promoter (from cauliflower mosaic virus), Mas-promoter, Mas-terminator, NOS-terminator and T-DNA border sequences (from Agrobacterium tumefaciens)} and Vector backbone region {ColE1 ori, GmR (from E. coli), pRi ori (from Agrobacterium tumefaciens)}. These nucleotides were located on the vector pCGN derivatives and introduced into the genome by the Ac-Ds transposon tagging system.
\n\u53c2\u8003\u6587\u732e\uff1a<\/strong>
\n\u2460Ito T., Motohashi R., Kuromori T., Mizukado S., Sakurai T., Kanahara H., Seki M. and Shinozaki K. (2002) Plant Physiol. 129: 1695-1699.12177482<\/a>
\n\u2461Kuromori T., Hirayama T., Kiyosue Y., Takabe H., Mizukado S., Sakurai T., Akiyama K., Kamiya A., Ito T. and Shinozaki K. (2004) Plant J. 37, 897-905.14996221<\/a>
\n\u2462Ito T., Motohashi R., Kuromori T., Noutoshi Y., Seki M., Kamiya A., Mizukado S., Sakurai T. and Shinozaki K. (2005) Plant Cell Physiol. 46: 1149-1153. 15840642<\/a><\/p>\n

 <\/p>\n

<\u30a2\u30af\u30c6\u30a3\u30d9\u30fc\u30b7\u30e7\u30f3\u30bf\u30b0\u30e9\u30a4\u30f3\u306e\u60c5\u5831\u306b\u3064\u3044\u3066><\/strong><\/p>\n

\u4f5c\u6210\u306b\u7528\u3044\u305f\u30d9\u30af\u30bf\u30fc\u540d\uff1a<\/strong>
\npPCVICEn4HPT\uff08Hayashi et al. Science 258, 1350-1353 (1992))
\n\u5bbf\u4e3b\u306b\u7d44\u307f\u8fbc\u307e\u308c\u305f\u907a\u4f1d\u5b50\u53ca\u3073\u7531\u6765\uff1a<\/strong>
\nT-DNA region {HPTII,Bla, ColE1 ori (from E.coli), NOS promoter, poly(A) signal from gene 4, T-DNA border sequence (from Agrobacterium tumefaciens), CaMV 35S RNA promoter\u00a0 (from Cauliflower mosaic virus\uff08CaMV))} and Vector backbone region {oriV, oriT (from Pseudomonas aruginosa)} These nucleotides are located on the vector pPCVICEn4HPT.
\n\u53c2\u8003\u6587\u732e\uff1a<\/strong>
\n\u2460Hayashi H, Czaja I, Lubenow H, Schell J, Walden R. (1992) Science 258, 1350-1353 PMID: 1455228<\/a>
\n\u2461\u67ff\u672c\u8fb0\u7537\u3001\u30a2\u30af\u30c6\u30a3\u30d9\u30fc\u30b7\u30e7\u30f3\u30bf\u30ae\u30f3\u30b0\u6cd5\u3001\u690d\u7269\u7d30\u80de\u5de5\u5b66\u30b7\u30ea\u30fc\u30ba\u3000\uff14\u3000\u30e2\u30c7\u30eb\u690d\u7269\u306e\u5b9f\u9a13\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\uff08\u79c0\u6f64\u793e\uff09pp135-142 (1996)<\/p>\n

 <\/p>\n

<FOX\u30e9\u30a4\u30f3\uff08\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u907a\u4f1d\u5b50\uff09\u306e\u60c5\u5831\u306b\u3064\u3044\u3066><\/strong><\/p>\n

\u4f5c\u6210\u306b\u7528\u3044\u305f\u30d9\u30af\u30bf\u30fc\u540d\uff1a<\/strong>pBIG2113SF
\n\u5bbf\u4e3b\u306b\u7d44\u307f\u8fbc\u307e\u308c\u305f\u907a\u4f1d\u5b50\u53ca\u3073\u7531\u6765\uff1a<\/strong>
\nT-DNA region {HPT gene (resistant to Hygromycin)(from Escherichia coli), CAMV 35S promoter (from cauliflower mosaic virus), NOS promoter, NOS terminator, gene7 promoter, T-DNA border sequences (from Agrobacterium tumefaciens), full-length cDNA (from Arabidopsis thaliana), modified pBluescript < Bla(resistant to Ampicillin)(\u03b2-lactamase gene), ColE1 ori, Lac promoter (from Escherichia coli), f1 ori (from Enterobacteriaphage F1), T7 promoter (from Enterobacteriaphage T7), and T3 promoter (from Enterobacteriaphage T3) >} and Vector backbone region {TetR (resistant to Tetracycline), NPTIII(resistant to Kanamycin), oriT, traJ, oriV, IS1, trfA (from Escherichia coli)}. These nucleotides are located on the vector pBIG2113SF derivative.\u3000
\n\u53c2\u8003\u6587\u732e\uff1a<\/strong>
\nIchikawa T, Nakazawa M, Kawashima M,Iizumi H, Kuroda H, Kondou Y, Tuhara Y, Suzuki K, Ishikawa A, Seki M, Fujita M, Motohashi R, Nagata N, Takagi T, Shinozaki K and Matsui M (2006) Plant J. 48:974-985 PMID: 17227551<\/a><\/p>\n

 <\/p>\n

<FOX\u30e9\u30a4\u30f3\uff08\u30a4\u30cd\u907a\u4f1d\u5b50\uff09\u306e\u60c5\u5831\u306b\u3064\u3044\u3066><\/strong><\/p>\n

\u4f5c\u6210\u306b\u7528\u3044\u305f\u30d9\u30af\u30bf\u30fc\u540d\uff1a<\/strong>pBIG2113SF
\n\u5bbf\u4e3b\u306b\u7d44\u307f\u8fbc\u307e\u308c\u305f\u907a\u4f1d\u5b50\u53ca\u3073\u7531\u6765\uff1a<\/strong>
\n\u30cf\u30a4\u30b0\u30ed\u30de\u30a4\u30b7\u30f3\u8010\u6027\u907a\u4f1d\u5b50\uff08\u5927\u8178\u83cc\u7531\u6765\uff09\u300135S\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff08\u30ab\u30ea\u30d5\u30e9\u30ef\u30fc\u30e2\u30b6\u30a4\u30af\u30a6\u30a4\u30eb\u30b9\u7531\u6765\uff09\u3001nos\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3001nos\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\u3001T-DNA\u30dc\u30fc\u30c0\u30fc\u9818\u57df\u3001gene7 polyA signal\uff08\u4ee5\u4e0a\u30a2\u30b0\u30ed\u30d0\u30af\u30c6\u30ea\u30a6\u30e0\u7531\u6765\uff09\u3001\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u8010\u6027\u907a\u4f1d\u5b50, ColE1, Lac\u30d7\u30ed\u30e2\u30fc\u30bf\uff08\u5927\u8178\u83cc\u7531\u6765\uff09\u3001T3\u30d7\u30ed\u30e2\u30fc\u30bf\uff08\u30dd\u30c9\u30a6\u30a4\u30eb\u30b9\u79d1\uff0dT3\u30d5\u30a1\u30fc\u30b8\u7531\u6765\uff09\u3001T7\u30d7\u30ed\u30e2\u30fc\u30bf\uff08\u30dd\u30c9\u30a6\u30a4\u30eb\u30b9\u79d1\uff0dT7\u30d5\u30a1\u30fc\u30b8\u7531\u6765\uff09\u3001F1\u30aa\u30ea\uff08\u30a4\u30ce\u30a6\u30a4\u30eb\u30b9\u79d1-F1 phage\u7531\u6765\uff09\u3001\u5b8c\u5168\u9577cDNA\uff08\u30a4\u30cd\u7531\u6765\uff09\u3000
\n\u53c2\u8003\u6587\u732e\uff1a<\/strong>
\nIchikawa T, Nakazawa M, Kawashima M,Iizumi H, Kuroda H, Kondou Y, Tuhara Y, Suzuki K, Ishikawa A, Seki M, Fujita M, Motohashi R, Nagata N, Takagi T, Shinozaki K and Matsui M (2006) Plant J. 48:974-985 PMID: 17227551<\/a><\/p>\n

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<\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u8ee2\u5199\u56e0\u5b50-\u30b0\u30eb\u30b3\u30b3\u30eb\u30c1\u30b3\u30a4\u30c9\u53d7\u5bb9\u4f53\u7cfb\u7d71\uff08TF-GR\uff09 \u7a2e\u5b50\u306e\u60c5\u5831\u306b\u3064\u3044\u3066><\/strong><\/p>\n

\u4f5c\u6210\u306b\u7528\u3044\u305f\u30d9\u30af\u30bf\u30fc\u540d\uff1a<\/strong>pGWTF-GR vector
\n\u5bbf\u4e3b\u306b\u7d44\u307f\u8fbc\u307e\u308c\u305f\u907a\u4f1d\u5b50\u53ca\u3073\u7531\u6765\uff1a<\/strong>
\nNPTII\uff08\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u8010\u6027\uff09\u907a\u4f1d\u5b50\uff08\u5927\u8178\u83cc\u7531\u6765\uff09\u3001att\u914d\u5217\uff08\u03bb\u30d5\u30a1\u30fc\u30b8\u7531\u6765\uff09\u3001CaMV 35S \u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff08\u30ab\u30ea\u30d5\u30e9\u30ef\u30fc\u30e2\u30b6\u30a4\u30af\u30a6\u30a3\u30eb\u30b9\u7531\u6765\uff09\u3001\u30b0\u30eb\u30b3\u30b3\u30eb\u30c1\u30b3\u30a4\u30c9\u53d7\u5bb9\u4f53\uff08GR\uff09\uff08Rat\u7531\u6765\uff09nos\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3001nos\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\u3001T-DNA\u30dc\u30fc\u30c0\u30fc\u9818\u57df\uff08\u30a2\u30b0\u30ed\u30d0\u30af\u30c6\u30ea\u30a6\u30e0\u7531\u6765\uff09\u3001\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u8ee2\u5199\u56e0\u5b50\u907a\u4f1d\u5b50\uff08TF)\uff08\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u7531\u6765\uff09
\n\u53c2\u8003\u6587\u732e\uff1a<\/strong>
\nShimada S, Yanagawa Y, Munesada T, Horii Y, Kuriyama T, Kawashima M, Kondou Y, Yoshizumi T, Mitsuda N, Ohme-Takagi M, Makita Y, Matsui M. A collection of inducible transcription factor-glucocorticoid receptor fusion lines for functional analyses in Arabidopsis thaliana. Plant J. 2022 Jul;111(2):595-607. doi: 10.1111\/tpj.15796. Epub 2022 May 21. PMID: 35510416<\/a><\/p>\n

 <\/p>\n

<\u8449\u7dd1\u4f53\u95a2\u9023\u907a\u4f1d\u5b50\u7834\u58ca\u7cfb\u7d71\u7a2e\u5b50\u306e\u60c5\u5831\u306b\u3064\u3044\u3066><\/strong><\/p>\n

\u4f5c\u6210\u306b\u7528\u3044\u305f\u30d9\u30af\u30bf\u30fc\u540d\uff1a<\/strong>pCGN derivatives (psy00001-00701), pROK2 (psy01001-02804)
\n\u5bbf\u4e3b\u306b\u7d44\u307f\u8fbc\u307e\u308c\u305f\u907a\u4f1d\u5b50\u53ca\u3073\u7531\u6765\uff1a<\/strong>
\n\u3010psy00001-00701\u3011
\nNPTII\uff08\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u8010\u6027\uff09\u907a\u4f1d\u5b50\u3001HPT\uff08aph4\uff09\uff08\u30cf\u30a4\u30b0\u30ed\u30de\u30a4\u30b7\u30f3\u8010\u6027\uff09\u907a\u4f1d\u5b50\u3001GUS\u907a\u4f1d\u5b50\uff08\u4ee5\u4e0a\u5927\u8178\u83cc\u7531\u6765\uff09\u3001ALS\uff08\u30af\u30ed\u30ed\u30b9\u30eb\u30d5\u30ed\u30f3\u8010\u6027\uff09\u907a\u4f1d\u5b50\uff08\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u7531\u6765\uff09\u3001Ds element\uff08\u30c8\u30a6\u30e2\u30ed\u30b3\u30b7\u7531\u6765\uff09\u3001CaMV 35S RNA \u907a\u4f1d\u5b50\u306e5\u2019\u5236\u5fa1\u9818\u57df\u3001CaMV 19S RNA \u907a\u4f1d\u5b50\u306e 5\u2019\u5236\u5fa1\u9818\u57df\uff08\u4ee5\u4e0a\u30ab\u30ea\u30d5\u30e9\u30ef\u30fc\u30e2\u30b6\u30a4\u30af\u30a6\u30a3\u30eb\u30b9\u7531\u6765\uff09\u3001mas\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3001mas\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\u3001nos\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\u3001T-DNA\u30dc\u30fc\u30c0\u30fc\u9818\u57df\uff08\u4ee5\u4e0a\u30a2\u30b0\u30ed\u30d0\u30af\u30c6\u30ea\u30a6\u30e0\u7531\u6765\uff09
\n\u3010psy01001-02804\u3011
\nNPTII\uff08\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u8010\u6027\uff09\u907a\u4f1d\u5b50\uff08\u5927\u8178\u83cc\u7531\u6765\uff09\u3001CaMV 35S RNA \u907a\u4f1d\u5b50\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff08\u30ab\u30ea\u30d5\u30e9\u30ef\u30fc\u30e2\u30b6\u30a4\u30af\u30a6\u30a3\u30eb\u30b9\u7531\u6765\uff09\u3001nos\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3001nos\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\u3001T-DNA\u30dc\u30fc\u30c0\u30fc\u9818\u57df\uff08\u4ee5\u4e0a\u30a2\u30b0\u30ed\u30d0\u30af\u30c6\u30ea\u30a6\u30e0\u7531\u6765\uff09
\n\u53c2\u8003\u6587\u732e\uff1a<\/strong>
\nMyouga F, Akiyama K, Tomonaga Y, Kato A, Sato Y, Kobayashi M, Nagata N, Sakurai T, Shinozaki K. The Chloroplast Function Database II: a comprehensive collection of homozygous mutants and their phenotypic\/genotypic traits for nuclear-encoded chloroplast proteins. Plant Cell Physiol. 2013 Feb;54(2):e2. doi: 10.1093\/pcp\/pcs171. Epub 2012 Dec 10. PubMed PMID: 23230006<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

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