{"id":5856,"date":"2024-08-28T09:00:31","date_gmt":"2024-08-28T00:00:31","guid":{"rendered":"http:\/\/epd.brc.riken.jp\/en\/?p=5856"},"modified":"2024-08-28T09:07:45","modified_gmt":"2024-08-28T00:07:45","slug":"new-release-transgenic-by-2-cell-lines-2","status":"publish","type":"post","link":"http:\/\/epd.brc.riken.jp\/en\/archives\/5856","title":{"rendered":"New Release: Transgenic BY-2 Cell Lines"},"content":{"rendered":"<p>We are pleased to announce the release of three plant cell lines.<br \/>\nFor inquiries, please contact us at <a href=\"mailto:plant.brc@riken.jp\">plant.brc@riken.jp<\/a>.<\/p>\n<p>1. rpc00092: Tobacco TBY2-31\/ST(E)<br \/>\nThis transgenic BY-2 cell line expresses estradiol-inducible GFP-AtSYP31 and estradiol-inducible ST-mRFP, which visualize the cis- and trans-Golgi, respectively.<br \/>\nThe cells are maintained in a suspension culture in liquid medium.<\/p>\n<p>DOI: <a href=\"https:\/\/doi.org\/10.1242\/jcs.203893\">10.1242\/jcs.203893<\/a><br \/>\n<a href=\"https:\/\/plant.rtc.riken.jp\/resource\/cell_line\/cell_line_detail.html?brcno=rpc00092\">Exp-Plant Catalog<\/a><\/p>\n<p>2. rpc00097: Tobacco TBY2-31\/41<br \/>\nThis transgenic BY-2 cell line expresses GFP-AtSYP31 and mRFP-AtSYP41, which visualize the cis-Golgi and trans-Golgi network, respectively.<br \/>\nThe cells are maintained in a suspension culture in liquid medium.<\/p>\n<p>DOI: <a href=\"https:\/\/doi.org\/10.1093\/pcp\/pcx028\">10.1093\/pcp\/pcx028<\/a><br \/>\n<a href=\"https:\/\/plant.rtc.riken.jp\/resource\/cell_line\/cell_line_detail.html?brcno=rpc00097\">Exp-Plant Catalog<\/a><\/p>\n<p>3. rpc00098: Tobacco topless3-GFP<br \/>\nThis transgenic BY-2 cell line expresses topless3-GFP.<br \/>\nThe cells are maintained in a callus culture on gellan gum medium.<\/p>\n<p>DOI: <a href=\"https:\/\/doi.org\/10.1074\/jbc.RA118.005843\">https:\/\/doi.org\/10.1074\/jbc.RA118.005843<\/a><br \/>\n<a href=\"https:\/\/plant.rtc.riken.jp\/resource\/cell_line\/cell_line_detail.html?brcno=rpc00098\">Exp-Plant Catalog<\/a><\/p>\n<p>License Agreement<br \/>\nThe mRFP gene used in these plant bioresources was provided by Professor Roger Y. Tsien at the University of California San Diego (UCSD).<br \/>\nIt is necessary to obtain permission by using UCSD\u2019s MTA.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>We are pleased to announce the release of three plant cell lines. For inquiries, please contact us at plant.brc@riken.jp. 1. rpc00092: Tobacco TBY2-31\/ST(E) This transgenic BY-2 cell line expresses estradiol-inducible GFP-AtSYP31 and estradiol-inducible ST-mRFP, which visualize the cis- and trans-Golgi, respectively. The cells are maintained in a suspension culture in liquid medium. DOI: 10.1242\/jcs.203893 Exp-Plant Catalog 2. rpc00097: Tobacco TBY2-31\/41 This transgenic BY-2 cell line expresses GFP-AtSYP31 and mRFP-AtSYP41, which visualize the cis-Golgi and trans-Golgi network, respectively. The cells are maintained in a suspension culture in liquid medium. DOI: 10.1093\/pcp\/pcx028 Exp-Plant Catalog 3. rpc00098: Tobacco topless3-GFP This transgenic BY-2 cell line expresses topless3-GFP. The cells are maintained in a callus culture on gellan gum medium. DOI: https:\/\/doi.org\/10.1074\/jbc.RA118.005843 Exp-Plant Catalog License Agreement The mRFP gene used in these plant bioresources was provided by Professor Roger Y. Tsien at the University of California San Diego (UCSD). It is necessary to obtain permission by using UCSD\u2019s MTA.<\/p>\n","protected":false},"author":17,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_seopress_robots_primary_cat":"none","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"categories":[15],"tags":[],"class_list":["post-5856","post","type-post","status-publish","format-standard","hentry","category-news"],"_links":{"self":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/posts\/5856","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/17"}],"replies":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=5856"}],"version-history":[{"count":1,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/posts\/5856\/revisions"}],"predecessor-version":[{"id":5859,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/posts\/5856\/revisions\/5859"}],"wp:attachment":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=5856"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/categories?post=5856"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/tags?post=5856"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}