{"id":4267,"date":"2015-11-18T14:21:14","date_gmt":"2015-11-18T05:21:14","guid":{"rendered":"http:\/\/epd.brc.riken.jp\/en\/?page_id=4267"},"modified":"2023-10-06T11:04:06","modified_gmt":"2023-10-06T02:04:06","slug":"cell","status":"publish","type":"page","link":"http:\/\/epd.brc.riken.jp\/en\/brachypodium\/cell","title":{"rendered":"Brachypodium embryogenic callus (domestic only)"},"content":{"rendered":"<p><strong>Resource number:<\/strong>\u00a0rpc10001<\/p>\n<p><strong>Species name:<\/strong>\u00a0<a href=\"https:\/\/epd.brc.riken.jp\/en\/brachypodium\/bd21\" rel=\"noopener\" target=\"_blank\"><em>Brachypodium distachyon<\/em> accession Bd21<\/a><\/p>\n<p><strong>Starting material:<\/strong> Immature embryos<\/p>\n<p><strong>Characteristic feature:<\/strong> Embryogenic callus suitable for the regeneration of transgenic plants by using the Agrobacterium-mediated method or the particle bombardment technique<\/p>\n<p><strong>Culture conditions:<\/strong> Callus induction medium 2, 27\u2103 (lower limit 25\u2103), dark<\/p>\n<p><strong>Method for maintenance:<\/strong> Transfer a portion (approx. 2 mm in diameter) of growing callus to a fresh medium every 10 days. The growth of callus may be much slower under lower temperature such as 25\u2103. If it is the case, you would better wait for 14 days untill next subculture.<\/p>\n<p><strong>Protocols (in Japanese):<\/strong> <a href=\"https:\/\/epd.brc.riken.jp\/ja\/manual\/brachypodium_distachyon_howto\" rel=\"noopener\" target=\"_blank\">Handling techniques \u2013 Brachpodium distachyon<\/a><\/p>\n<li><a href=\"https:\/\/epd.brc.riken.jp\/ja\/wp-content\/uploads\/manual\/brachypodium_distachyon_howto_02-01.pdf\" rel=\"noopener\" target=\"_blank\">Transformation protocol, including culture media preparation<\/a><\/li>\n<li><a href=\"https:\/\/epd.brc.riken.jp\/ja\/wp-content\/uploads\/data\/RPC10001_comprs.pdf\" rel=\"noopener\" target=\"_blank\">Handling of cultured cells after arrival<\/a><\/li>\n<p><strong>Reference:<\/strong> Alves S, Worland B, Thole V, Snape JW, Bevan MW, Vain P (2009) A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21. Nature Protocols 4: 638\u2013649. DOI: <a href=\"https:\/\/doi.org\/10.1038\/nprot.2009.30\" rel=\"noopener\" target=\"_blank\">10.1038\/nprot.2009.30<\/a><\/p>\n<p><strong>Specific condition in MTA:<\/strong> None<\/p>\n<p><strong>Note:<\/strong> We recommend recipients to use the callus for transformation within one month after its arrival.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Resource number:\u00a0rpc10001 Species name:\u00a0Brachypodium distachyon accession Bd21 Starting material: Immature embryos Characteristic feature: Embryogenic callus suitable for the regeneration of transgenic plants by using the Agrobacterium-mediated method or the particle bombardment technique Culture conditions: Callus induction medium 2, 27\u2103 (lower limit 25\u2103), dark Method for maintenance: Transfer a portion (approx. 2 mm in diameter) of growing callus to a fresh medium every 10 days. The growth of callus may be much slower under lower temperature such as 25\u2103. If it is the case, you would better wait for 14 days untill next subculture. Protocols (in Japanese): Handling techniques \u2013 Brachpodium distachyon Transformation protocol, including culture media preparation Handling of cultured cells after arrival Reference: Alves S, Worland B, Thole V, Snape JW, Bevan MW, Vain P (2009) A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21. Nature Protocols 4: 638\u2013649. DOI: 10.1038\/nprot.2009.30 Specific condition in MTA: None Note: We recommend recipients to use the callus for transformation within one month after its arrival.<\/p>\n","protected":false},"author":17,"featured_media":0,"parent":3353,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-4267","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/4267","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/17"}],"replies":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=4267"}],"version-history":[{"count":2,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/4267\/revisions"}],"predecessor-version":[{"id":5707,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/4267\/revisions\/5707"}],"up":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3353"}],"wp:attachment":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=4267"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}