{"id":4109,"date":"2015-05-22T14:31:28","date_gmt":"2015-05-22T05:31:28","guid":{"rendered":"http:\/\/epd.brc.riken.jp\/en\/?page_id=4109"},"modified":"2023-05-31T13:27:13","modified_gmt":"2023-05-31T04:27:13","slug":"tac","status":"publish","type":"page","link":"http:\/\/epd.brc.riken.jp\/en\/pdna\/tac","title":{"rendered":"Arabidopsis TAC clones"},"content":{"rendered":"
\"Exp-Plant\u00a0Exp-Plant Catalog<\/a>\n<\/div>\n

This resource has been developed and deposited from Dr.\u00a0Daisuke Shibata (Kazusa DNA Research Institute).<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Resource number:<\/strong><\/td>\npdg00001-pdg10752<\/td>\n<\/tr>\n
Developer:<\/strong><\/td>\nDr. Daisuke Shibata (Kazusa DNA Research Institute)<\/td>\n<\/tr>\n
Total number of Clones:<\/strong><\/td>\n10,752<\/td>\n<\/tr>\n
Style of distribution:<\/strong><\/td>\nApprox. 100 ng of lyophilized plasmid DNA is provided.<\/td>\n<\/tr>\n
Number of clones available:<\/strong><\/td>\n10,752<\/td>\n<\/tr>\n
Organism:<\/strong><\/td>\nArabidopsis thaliana <\/em>accession Columbia<\/td>\n<\/tr>\n
Vector:<\/strong><\/td>\npYLTAC7<\/td>\n<\/tr>\n
Selection marker:<\/strong><\/td>\nkanamycin (for culturing E. coli and Agrobacterium) and hygromycin (for selecting transformed plants)<\/td>\n<\/tr>\n
Map and restriction site:<\/strong><\/td>\nHindIII, map and sequence for cloning site are available from Developer\u2019s site<\/a><\/td>\n<\/tr>\n
Specific feature:<\/strong><\/td>\nEnd-sequences of both NotI and SfiI sites have been characterized.<\/td>\n<\/tr>\n
Specific conditions for distribution:<\/strong><\/td>\nFollowing manuscript should be referred in the publication on a scientific journal.1.\u00a0Liu YG, Shirano Y, Fukaki H, Yanai Y, Tasaka M, Tabata S, Shibata D. Complementation of plant mutants with large genomic DNA fragments by atransformation-competent artificial chromosome vector accelerates positionalcloning. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6535-40. PubMed PMID:10339623<\/a><\/td>\n<\/tr>\n
Reference:<\/strong><\/td>\n1. Shibata D, Liu YG. Agrobacterium-mediated plant transformation with large DNA fragments. Trends Plant Sci. 2000 Aug;5(8):354-7. PubMed PMID:10908881<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

\u00a0Exp-Plant Catalog This resource has been developed and deposited from Dr.\u00a0Daisuke Shibata (Kazusa DNA Research Institute). Resource number: pdg00001-pdg10752 Developer: Dr. Daisuke Shibata (Kazusa DNA Research Institute) Total number of Clones: 10,752 Style of distribution: Approx. 100 ng of lyophilized plasmid DNA is provided. Number of clones available: 10,752 Organism: Arabidopsis thaliana accession Columbia Vector: pYLTAC7 Selection marker: kanamycin (for culturing E. coli and Agrobacterium) and hygromycin (for selecting transformed plants) Map and restriction site: HindIII, map and sequence for cloning site are available from Developer\u2019s site Specific feature: End-sequences of both NotI and SfiI sites have been characterized. Specific conditions for distribution: Following manuscript should be referred in the publication on a scientific journal.1.\u00a0Liu YG, Shirano Y, Fukaki H, Yanai Y, Tasaka M, Tabata S, Shibata D. Complementation of plant mutants with large genomic DNA fragments by atransformation-competent artificial chromosome vector accelerates positionalcloning. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6535-40. PubMed PMID:10339623 Reference: 1. Shibata D, Liu YG. Agrobacterium-mediated plant transformation with large DNA fragments. Trends Plant Sci. 2000 Aug;5(8):354-7. PubMed PMID:10908881<\/p>\n","protected":false},"author":17,"featured_media":0,"parent":3267,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"both","_seopress_redirections_param":"","_seopress_redirections_type":301,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-4109","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/4109","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/17"}],"replies":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=4109"}],"version-history":[{"count":11,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/4109\/revisions"}],"predecessor-version":[{"id":5632,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/4109\/revisions\/5632"}],"up":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3267"}],"wp:attachment":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=4109"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}