{"id":3333,"date":"2014-05-27T16:07:28","date_gmt":"2014-05-27T07:07:28","guid":{"rendered":"http:\/\/plant1.epd.rbrc.jp\/en\/?page_id=3333"},"modified":"2026-03-02T11:43:34","modified_gmt":"2026-03-02T02:43:34","slug":"pcellc","status":"publish","type":"page","link":"http:\/\/epd.brc.riken.jp\/en\/pcellc","title":{"rendered":"Plant Cultured Cell Resources"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/botg1.png\" alt=\"Exp-Plant Catalog \u2013 Plant Cell Lines\" width=\"11\" height=\"12\" \/> <a href=\"https:\/\/plant.rtc.riken.jp\/resource\/cell_line\/cell_line_list.html\" rel=\"noopener noreferrer\" target=\"_blank\"><strong>Database: Exp-Plant Catalog \u2013 Plant Cell Lines<\/strong><\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/botg1.png\" alt=\"RIKEN BRC plant cell line documentation\" width=\"11\" height=\"12\" \/> <a href=\"https:\/\/plant.rtc.riken.jp\/resource\/cell_line\/web_documents\/index.html\" rel=\"noopener noreferrer\" target=\"_blank\"><strong>On-line manual: RIKEN BRC plant cell line documentation<\/strong><\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/botg1.png\" alt=\"List of plant cell lines \" width=\"11\" height=\"12\" \/> <a href=\"https:\/\/epd.brc.riken.jp\/en\/wp-content\/uploads\/data\/RIKEN_BRC_plant_cell_list_20260304.pdf\" rel=\"noopener noreferrer\" target=\"_blank\"><strong>List of plant cell lines <\/strong><img decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/pdficon_s.png\" border=\"0\" \/><\/a><\/p>\n<div style=\"background-color: #e1f6dd; position: relative; top: 10px; border: 1px solid #cccccc; \">\n<div style=\"height: 10px; \">\n<\/div>\n<div style=\"display: inline-block; text-align: center; width: 200px; vertical-align: top; \">\n<a href=\"\/en\/wp-content\/uploads\/plant_cell_lines\/cryopreservation_protocol_en.pdf\" target=\"_blank\" data-wpel-link=\"internal\" rel=\"noopener noreferrer\"><img decoding=\"async\" alt=\"\" src=\"\/en\/wp-content\/uploads\/plant_cell_lines\/cryopreservation_protocol_en_thumbnail.jpg\" width=\"180\" style=\"border: solid 1px gray; \" \/><\/p>\n<p>Cryopreservation protocol <img decoding=\"async\" alt=\"\" src=\"\/ja\/wp-content\/uploads\/images\/pdficon_s.png\" border=\"0\" \/><\/a><\/p>\n<p><\/a><\/p>\n<\/div>\n<div style=\"clear:both\">\n<\/div>\n<div style=\"position: relative; left: 5px; top: 0px; \">\n<p><img loading=\"lazy\" decoding=\"async\" src=\"\/ja\/wp-content\/uploads\/images\/botg1.png\" alt=\"Technical Notes\" width=\"11\" height=\"12\" \/><a href=\"https:\/\/epd.brc.riken.jp\/en\/qa\/resource-handling\" target=\"_blank\" data-wpel-link=\"internal\" rel=\"noopener noreferrer\"> Technical Notes<\/a><\/p>\n<\/div>\n<\/div>\n<div style=\"height: 15px; \">\n<\/div>\n<hr>\n<section>\n<h6 class=\"t_style\">Plant cell line collection<\/h6>\n<p>Experimental Plant Division provides researchers with plant cell cultures that are useful for a wide range of plant research.<\/p>\n<ul>\n<li> Cultured cells of model plants<\/li>\n<li> Cell lines derived from secondary metabolite-producing plants<\/li>\n<li> Transgenic cell lines expressing fluorescent proteins<\/li>\n<\/ul>\n<p>Most of the cell lines are undifferentiated cell cultures that have lost capacity for differentiating into whole plants, except for carrot kurodagosun (<a href=\"https:\/\/plant.rtc.riken.jp\/resource\/cell_line\/web_documents\/cell_lines\/rpc00002.html\" data-wpel-link=\"external\" target=\"_blank\" rel=\"follow noopener noreferrer\" class=\"ext-link wpel-icon-right\"><span style=\"color:#006600;\">rpc00002<\/span><\/a>).<\/p>\n<\/section>\n<hr>\n<section>\n<h6 class=\"t_style\">Culture methods<\/h6>\n<p>The plant cell cultures have been maintained on semi-solid or liquid media by repeated subculturing at definite time intervals.<\/p>\n<p>Detailed culture methods are found in an on-line manual <a href=\"https:\/\/plant.rtc.riken.jp\/resource\/cell_line\/web_documents\/index.html\" data-wpel-link=\"external\" target=\"_blank\" rel=\"follow noopener noreferrer\" class=\"ext-link wpel-icon-right\"><span style=\"color:#006600;\">RIKEN BRC plant cell line documentation<\/span><\/a>.<\/p>\n<\/section>\n<hr>\n<section>\n<h6 class=\"t_style\">Ordering<\/h6>\n<p><b>Tobacco BY-2 and Arabidopsis T87<\/b><\/p>\n<p>Please send MTA and order form to customer support <a href=\"mailto:plant.brc@riken.jp\">plant.brc@riken.jp<\/a>.<\/p>\n<p><a href=\"https:\/\/epd.brc.riken.jp\/en\/distribution\/documents\" data-wpel-link=\"internal\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"color:#006600;\">Documents required for distribution<\/span><\/a> &lt;Tobacco BY-2 \/ Arabidopsis T87 cells&gt;<\/a><\/p>\n<p><b>Other cell lines<\/b><\/p>\n<p>Please contact customer support <a href=\"mailto:plant.brc@riken.jp\">plant.brc@riken.jp<\/a>.<\/p>\n<\/section>\n<hr>\n<section>\n<h6 class=\"t_style\">Shipping<\/h6>\n<p>Setting up schedule to ship orders is affected by the subculture cycle of plant cells or certain terms and conditions when they are shipped.<\/p>\n<ul>\n<li> Recipients are solely responsible for the customs clearance in accordance with laws of own country.<br \/>\nAsking your local courier for advice would be recommended.<\/li>\n<li> Plant cells are shipped by means of consignee payment.<br \/>\nFedEx is exclusively selected for the recipients in the States, but another choice is available in other areas.<\/li>\n<li> There are several transgenic cell lines in the stock, which cannot regenerate into whole plants under natural conditions.<br \/>\nIf any document relating to GMOs is required in the country\u2019s law and\/or the institute, please give the customer support a prior notice.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/epd.brc.riken.jp\/en\/distribution\" data-wpel-link=\"internal\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"color:#006600;\">Overseas Distribution<\/span><\/a><\/p>\n<p><a href=\"https:\/\/epd.brc.riken.jp\/en\/distribution#6\" data-wpel-link=\"internal\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"color:#006600;\">Fee Structure<\/span><\/a><\/p>\n<\/section>\n<hr>\n<section>\n<h6 class=\"t_style\">Delivering<\/h6>\n<p>Carefully handle the plant cells after they arrive.<\/p>\n<ul>\n<li> Be prepared with growth chambers, cell culture instruments and culture media.<\/li>\n<li> Transfer the cultured cells to fresh medium immediately.<\/li>\n<li> Storing the cultured cells in a refrigerator or a freezer must be avoided.<\/li>\n<\/ul>\n<p><div onclick=\"obj=document.getElementById('x1').style;obj.display=(obj.display=='none')?'block':'none';\">\n<a style=\"cursor:pointer;\" data-wpel-link=\"internal\">Suspension cultures<\/a><\/p>\n<\/div>\n<div id=\"x1\" style=\"display:none;clear:both;\">\n<ol>\n<li>Suspension cells are transferred onto semi-solid medium in a disposable flask.<\/li>\n<li>The flask is packed in a heat seal bag.<\/li>\n<li>The individually packed flasks are packaged in a Styroform container.<\/li>\n<\/ol>\n<\/div>\n<p><div onclick=\"obj=document.getElementById('x2').style;obj.display=(obj.display=='none')?'block':'none';\">\n<a style=\"cursor:pointer;\" data-wpel-link=\"internal\">Callus cultures<\/a><\/p>\n<\/div>\n<div id=\"x2\" style=\"display:none;clear:both;\">\n<ol>\n<li>Callus cells are placed on semi-solid medium in a plastic dish.<\/li>\n<li>The dish is packed in a heat seal bag.<\/li>\n<li>The individually packed dishes are packaged in a Styroform container.<\/li>\n<\/ol>\n<\/div>\n<\/section>\n<hr>\n<section>\n<h6 class=\"t_style\">Quality control<\/h6>\n<p>Experimental Plant Division has checked plant cell lines by PCR-based genotyping in order to authenticate their identity.<\/p>\n<ul>\n<li> Identification of the original plant species (once a year)<\/li>\n<li> Detection of inserted DNA (if any, once a year)<\/li>\n<\/ul>\n<p><a href=\"https:\/\/epd.brc.riken.jp\/en\/quality_control#qc4\" data-wpel-link=\"internal\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"color:#006600;\">Quality Control (4) Plant cultured cells<\/span><\/a><\/p>\n<\/section>\n<hr>\n<section>\n<h6 class=\"t_style\">Citation of cell lines<\/h6>\n<p>When results obtained by using the plant cell lines are published in a scientific journal, it should be cited in the following manner:<\/p>\n<blockquote><p>\n<i>Nicotiana tabacum<\/i> BY-2 cell line (rpc00001) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan.\n<\/blockquote>\n<\/section>\n","protected":false},"excerpt":{"rendered":"<p>Database: Exp-Plant Catalog \u2013 Plant Cell Lines On-line manual: RIKEN BRC plant cell line documentation List of plant cell lines Cryopreservation protocol Technical Notes Plant cell line collection Experimental Plant Division provides researchers with plant cell cultures that are useful for a wide range of plant research. Cultured cells of model plants Cell lines derived from secondary metabolite-producing plants Transgenic cell lines expressing fluorescent proteins Most of the cell lines are undifferentiated cell cultures that have lost capacity for differentiating into whole plants, except for carrot kurodagosun (rpc00002). Culture methods The plant cell cultures have been maintained on semi-solid or liquid media by repeated subculturing at definite time intervals. Detailed culture methods are found in an on-line manual RIKEN BRC plant cell line documentation. Ordering Tobacco BY-2 and Arabidopsis T87 Please send MTA and order form to customer support plant.brc@riken.jp. Documents required for distribution &lt;Tobacco BY-2 \/ Arabidopsis T87 cells&gt; Other cell lines Please contact customer support plant.brc@riken.jp. Shipping Setting up schedule to ship orders is affected by the subculture cycle of plant cells or certain terms and conditions when they are shipped. Recipients are solely responsible for the customs clearance in accordance with laws of own country. Asking your [&hellip;]<\/p>\n","protected":false},"author":11,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_robots_primary_cat":"","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-3333","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3333","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=3333"}],"version-history":[{"count":21,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3333\/revisions"}],"predecessor-version":[{"id":6059,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/3333\/revisions\/6059"}],"wp:attachment":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=3333"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}