{"id":3275,"date":"2014-05-27T11:18:43","date_gmt":"2014-05-27T02:18:43","guid":{"rendered":"http:\/\/plant1.epd.rbrc.jp\/en\/?page_id=3275"},"modified":"2023-05-31T13:22:26","modified_gmt":"2023-05-31T04:22:26","slug":"rafl_clones","status":"publish","type":"page","link":"http:\/\/epd.brc.riken.jp\/en\/pdna\/rafl_clones","title":{"rendered":"RIKEN Arabidopsis full-length cDNA clones (RAFL clones)"},"content":{"rendered":"
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\"Exp-Plant\u00a0Exp-Plant Catalog<\/a>\n<\/div>\n

This resource is called as RIKEN Arabidopsis full-length cDNA clones (RAFL clones) that has been developed by RIKEN Genomic Sciences Center (GSC) in Yokohama, Japan. The clones were deposited from Dr. Kazuo Shinozaki who is a director of RIKEN Plant Science Center (PSC) and a former group director of Plant Functional Genomics Research Group in RIKEN GSC. The RIKEN GSC, SSP (Salk\/Stanford\/PGEC) consortium and National Institute of Genetics (NIG) have characterized full-length sequence of approx. 18,000 RAFL clones with the supports from NSF 2010 Project and National BioResource Project. By the SSP consortium, the RAFL clones are called as \u2018R clone\u2019 and distinguished from \u2018U clone\u2019 that harbors ORF sequence of R clone in pUNI vector.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Resource number:<\/strong><\/td>\npda00001- (with full-length sequence),
\npdx00001-\/pdy00001-\/pdz00001- (with 5\u2019-and\/or 3\u2019-end sequences)<\/td>\n<\/tr>\n
Developer:<\/strong><\/td>\nDrs. Motoaki Seki and *Kazuo Shinozaki (RIKEN GSC, *RIKEN PSC).<\/td>\n<\/tr>\n
Total number of clones:<\/strong><\/td>\napprox. 18,000 (pda), 220,000 (pdx\/pdy\/pdz)<\/td>\n<\/tr>\n
Style of distribution:<\/strong><\/td>\nApprox. 100 ng of lyophilized plasmid DNA is provided.<\/td>\n<\/tr>\n
Number of\u00a0available<\/strong>\u00a0clones:<\/strong><\/td>\n16,915 clones (pda), 228,886 clones (pdx\/pdy\/pdz) (Mar. 2006)<\/td>\n<\/tr>\n
Background ecotype:<\/strong><\/td>\nColumbia<\/td>\n<\/tr>\n
Vector:<\/strong><\/td>\nmodified pBluescript<\/td>\n<\/tr>\n
Selection marker:<\/strong><\/td>\nampicillin<\/td>\n<\/tr>\n
Map and restriction site:<\/strong><\/td>\nShown\u00a0here<\/a><\/td>\n<\/tr>\n
Specific feature:<\/strong><\/td>\nFull-length sequence and\/or 5\u2019-\/3\u2019-end sequences have been characterized.<\/td>\n<\/tr>\n
Specific conditions for distribution:<\/strong><\/td>\n<\/tr>\n
(a)The RECIPIENT agrees to expressly describe that “the Arabidopsis full-length cDNA clone was developed by the plant genome project of RIKEN Genomic Sciences Center” in any publication reporting the use thereof.<\/td>\n<\/tr>\n
(b)The manuscripts listed below should be referred in the publication on scientific journal.<\/td>\n<\/tr>\n
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  1. Seki M, Carninci P, Nishiyama Y, Hayashizaki Y and Shinozaki K (1998) High-efficiency cloning of Arabidopsis full-length cDNA by biotinylated CAP trapper. Plant Journal 15: 707-720.<\/span><\/li>\n
  2. Seki M, Narusaka M, Kamiya A, Ishida J, Satou M, Sakurai T, Nakajima M, Enju A, Akiyama K, Oono Y, Muramatsu M, Hayashizaki Y, Kawai J, Carninci P, Itoh M, Ishii Y, Arakawa T, Shibata K, Shinagawa A and Shinozaki K (2002) Functional Annotation of a Full-Length Arabidopsis cDNA Collection. Science 296: 141-145.<\/span><\/li>\n<\/ol>\n<\/td>\n<\/tr>\n
Reference:<\/strong><\/td>\n<\/tr>\n
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  1. Seki, M., Carninci, P., Nishiyama, Y., Hayashizaki, Y. and Shinozaki, K. (1998) Plant Journal 15: 707-720. PMID:\u00a0<\/span>9778851<\/a><\/li>\n
  2. Seki, M., Narusaka, M., Kamiya, A., Ishida, J., Satou, M., Sakurai, T., Nakajima, M., Enju, A., Akiyama, K., Oono, Y., Muramatsu, M., Hayashizaki, Y., Kawai, J., Carninci, P., Itoh, M., Ishii, Y., Arakawa, T., Shibata, K., Shinagawa, A. and Shinozaki, K. (2002) Science 296: 141-145. PMID:\u00a011910074<\/a><\/li>\n
  3. Sakurai T, Satou M, Akiyama K, Iida K, Seki M, Kuromori T, Ito T, Konagaya A, Toyoda T, and Shinozaki K. (2005) Nucleic Acids Res. 33(Database issue): D647-50. PMID:\u00a015608280<\/a><\/li>\n<\/ol>\n<\/td>\n<\/tr>\n
\u00a0NOTE:<\/strong><\/td>\n<\/tr>\n
\u00a0(1)Detailed information including full-length sequence of the individual RAFL clone is available from the following websites:\n