{"id":3191,"date":"2014-05-22T16:53:02","date_gmt":"2014-05-22T07:53:02","guid":{"rendered":"http:\/\/plant1.epd.rbrc.jp\/en\/?page_id=3191"},"modified":"2026-04-06T15:43:49","modified_gmt":"2026-04-06T06:43:49","slug":"arabidopsis_poolset","status":"publish","type":"page","link":"http:\/\/epd.brc.riken.jp\/en\/seed\/arabidopsis_poolset","title":{"rendered":"Pools of Arabidopsis natural accessions"},"content":{"rendered":"

The resource is distributed as eight individual pools (tubes), each containing 50 Arabidopsis natural accessions, resulting in a total of 400 accessions for screening purposes.
\nGenotyping service is available after screening.<\/p>\n

Information for the resource:<\/p>\n

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  1. We propagated natural accessions with single seed descent (SSD) method. We characterized genotype of each accession using SSR marker. We also obtained 149 SNPs data from the 3rd generation of each accession. Seeds of 2nd<\/sup>\u00a0or 3rd<\/sup>\u00a0generation were used for this resource.<\/li>\n
  2. From each accession, 400 seeds were sampled. The seeds from every 50 accessions were mixed to give 8 stocks in total. Then 400 seeds were sampled from each stock to create 8 pools for screening use.<\/li>\n
  3. RECIPINT of the resource can apply to the genotyping service after screening. Using SSR marker, we can identify the accession of the plants of your interest.<\/li>\n<\/ol>\n

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    Please note:<\/p>\n