{"id":2686,"date":"2014-05-21T15:00:03","date_gmt":"2014-05-21T06:00:03","guid":{"rendered":"http:\/\/plant1.epd.rbrc.jp\/en\/?page_id=2686"},"modified":"2017-07-25T10:32:00","modified_gmt":"2017-07-25T01:32:00","slug":"rafl","status":"publish","type":"page","link":"http:\/\/epd.brc.riken.jp\/en\/qa\/rafl","title":{"rendered":"Maps for RAFL clones"},"content":{"rendered":"<p>RAFL clones have been constructed with several vector systems.\u00a0\u03bbZAP was used for RAFL02~RAFL06,\u00a0\u03bbFLC-1-B for RAFL07~RAFL11,RAFL26 and\u00a0\u03bbFLC-1-E for RAFL12~RAFL25.<br \/>\nWe send RAFL clone as plasmid DNA that was excised from the phage vector mentioned above. Selection marker is ampicillin.<\/p>\n<p>&nbsp;<\/p>\n<p>Maps for RAFL clones:<\/p>\n<p style=\"padding-left: 30px;\"><a href=\"\/en\/qa\/rafl\/rafl2\">RAFL02~RAFL03<\/a><br \/>\n<a href=\"\/en\/qa\/rafl\/rafl4\">RAFL04~RAFL06<\/a><br \/>\n<a href=\"\/en\/qa\/rafl\/rafl7\">RAFL07~11,RAFL26<\/a><br \/>\n<a href=\"\/en\/qa\/rafl\/rafl12\">RAFL12~RAFL25<\/a><\/p>\n<p>&nbsp;<br \/>\nReferences: M. Seki et al., Science 296, 141 (2002);<br \/>\nP.Carninci et al., Genomics 77, 79 (2001).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>RAFL clones have been constructed with several vector systems.\u00a0\u03bbZAP was used for RAFL02~RAFL06,\u00a0\u03bbFLC-1-B for RAFL07~RAFL11,RAFL26 and\u00a0\u03bbFLC-1-E for RAFL12~RAFL25. We send RAFL clone as plasmid DNA that was excised from the phage vector mentioned above. Selection marker is ampicillin. &nbsp; Maps for RAFL clones: RAFL02~RAFL03 RAFL04~RAFL06 RAFL07~11,RAFL26 RAFL12~RAFL25 &nbsp; References: M. Seki et al., Science 296, 141 (2002); P.Carninci et al., Genomics 77, 79 (2001).<\/p>\n","protected":false},"author":11,"featured_media":0,"parent":2886,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"","_seopress_robots_follow":"","_seopress_robots_imageindex":"","_seopress_robots_snippet":"","_seopress_robots_primary_cat":"","_seopress_robots_breadcrumbs":"","_seopress_robots_freeze_modified_date":"","_seopress_robots_custom_modified_date":"","_seopress_robots_canonical":"","_seopress_social_fb_title":"","_seopress_social_fb_desc":"","_seopress_social_fb_img":"","_seopress_social_fb_img_attachment_id":0,"_seopress_social_fb_img_width":0,"_seopress_social_fb_img_height":0,"_seopress_social_twitter_title":"","_seopress_social_twitter_desc":"","_seopress_social_twitter_img":"","_seopress_social_twitter_img_attachment_id":0,"_seopress_social_twitter_img_width":0,"_seopress_social_twitter_img_height":0,"_seopress_redirections_value":"","_seopress_redirections_enabled":"","_seopress_redirections_enabled_regex":"","_seopress_redirections_logged_status":"","_seopress_redirections_param":"","_seopress_redirections_type":0,"_seopress_analysis_target_kw":"","footnotes":"","_wp_rev_ctl_limit":""},"class_list":["post-2686","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/2686","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/comments?post=2686"}],"version-history":[{"count":5,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/2686\/revisions"}],"predecessor-version":[{"id":4603,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/2686\/revisions\/4603"}],"up":[{"embeddable":true,"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/pages\/2886"}],"wp:attachment":[{"href":"http:\/\/epd.brc.riken.jp\/en\/wp-json\/wp\/v2\/media?parent=2686"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}