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シロイヌナズナ完全長 cDNA クローンの取り扱い方

RAFL clones have been constructed with several vector systems. λZAP was used for RAFL2-6, λFLC-1-B for RAFL7-11 and λFLC-1-E for RAFL12-21.
We send RAFL clone as plasmid DNA that was excised from the phage vector mentioned above. Selection marker is ampicillin.

 

Maps for RAFL clones:
RAFL 2-3
RAFL 4-6
RAFL 7-11,26
RAFL 12-25

References: M. Seki et al., Science 296, 141 (2002);
P. Carninci et al., Genomics 77, 79 (2001).