Experimental Plant Division

This resource has been developed by the collaboration of National Institute for Basic Biology (NIBB) and RIKEN GSC, and the sequence was characterized at National Institute of Genetics (NIG). The clones were deposited from Prof. Mitsuyasu Hasebe, Division of Speciation Mechanisms 2 and Computer Laboratory, NIBB in Okazaki, Japan.

Resource number:	pdp00001-, pdr00001-
Developer:	Dr. Mitsuyasu Hasebe (NIBB)
Total number of clones:	149,363
Style of distribution:	Approx. 100 ng of lyophilized plasmid DNA is provided.
Number of available clones:	149,363
Organism:	Physcomitrium patens (moss)
Vector:
The vector used for pdp77001-88219 (pphf) is pGCAPzf3, and that for pdp47001-76884/pdp89001-97804/pdr00001- (ppsp/ppaa/ppgs/ppls) is pME18S-FL3. The former vector is a derivative of pGCAP1 (Kato et al. DNA Research (2005); Accession No. AB191257), and the latter is on the catalogue by TOYOBO (Accession No. AB009864)). For other clones, lambda FLC-1-E is used. If you need further information, please inform us by e-mail.
Selection marker:	ampicillin
Map and restriction site:	For lambda FLC-1-E, shown here. For pME18S-FL3, please visit
manufacture’s HP
Specific feature:	5′- and 3′ -end sequences have been characterized.
Specific conditions for distribution:
When you publish your work using pdp77001-97804 (ppsp/pphf) and pdr00001- (ppls), please refer to the manuscript (2). For other clones, manuscript (1) should be referred. RECIPIENT can use the BIOLOGICAL MATERIAL for basic research only.
Reference:
Nishiyama T., Fujita T., Shin-I T., Seki M., Nishide H., Uchiyama I., Kamiya A., Carninci P., Hayashizaki Y., Shinozaki K., Kohara Y. and Hasebe M. (2003) Comparative genomics of Physcomitrella patens gametophytic transcriptome and Arabidopsis thaliana: implication for land plant evolution. PNAS 100 (13): 8007-8012. (PMID: 12808149) Rensing SA. et al. (2008) The Physcomitrella genome reveals evolutionary insights into the conquest of land by plants. Science 319(5859): 64-9. (PMID: 18079367)
NOTE:
Detailed information on the individual clone is available from the following website: http://moss.nibb.ac.jp